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Aplicación del método de coagulación de plasma al estudio ultraestructural de especímenes biológicos

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dc.creatorHernández Chavarría, Francisco
dc.creatorCoto, Erika
dc.creatorColmenares, Alirio
dc.creatorMoreira Carmona, Lisela
dc.date.accessioned2018-12-06T17:34:17Z
dc.date.available2018-12-06T17:34:17Z
dc.date.issued1991
dc.descriptionCopyright © 1991, Revista Biología Tropicales
dc.description.abstractBiological particulate specimens, including Saccharomyces cerevisiae yeast, bovine spermatozoa and human blood cells (normal erythrocytes and leukemic cells) were processed for scanning and transmission electron microscopy using the coagulated plasma technique. The specimens were suspended in frozen and thawed plasma; later, coagulation was induced by adding CaCI2. The clot was cut into small pieces and processed as tissue fragments. The technique is a useful tool when processing biological particulate specimens for electron microscopy.es
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes
dc.description.sponsorshipUniversidad de Costa Rica/[803-88-418]/UCR/Costa Ricaes
dc.identifier.citationhttps://revistas.ucr.ac.cr/index.php/rbt/article/view/24853
dc.identifier.codproyecto803-88418
dc.identifier.doihttps://doi.org/10.15517/RBT.V39I1.24853
dc.identifier.issn2215-2075
dc.identifier.urihttps://hdl.handle.net/10669/76248
dc.language.isoes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceRevista de Biología Tropical, vol. 39(1), pp. 177-180es
dc.subjectElectron microscopy techniquees
dc.subjectUltrastructure of biological particulate specimenses
dc.subject573.156 Plasmaes
dc.titleAplicación del método de coagulación de plasma al estudio ultraestructural de especímenes biológicoses
dc.typeartículo original

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Revista de Biología Tropical Vol.39 (1)