Aplicación del método de coagulación de plasma al estudio ultraestructural de especímenes biológicos
Fecha
1991
Tipo
artículo original
Autores
Hernández Chavarría, Francisco
Coto, Erika
Colmenares, Alirio
Moreira Carmona, Lisela
Título de la revista
ISSN de la revista
Título del volumen
Editor
Resumen
Biological particulate specimens, including Saccharomyces cerevisiae yeast, bovine spermatozoa and human blood cells (normal erythrocytes and leukemic cells) were processed for scanning and transmission electron microscopy using the coagulated plasma technique. The specimens were suspended in frozen and thawed plasma; later, coagulation was induced by adding CaCI2. The clot was cut into small pieces and processed as tissue fragments. The technique is a useful tool when processing biological particulate specimens for electron microscopy.
Descripción
Copyright © 1991, Revista Biología Tropical
Palabras clave
Electron microscopy technique, Ultrastructure of biological particulate specimens, 573.156 Plasma