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The genome of Epiperipatus biolleyi (Peripatidae), a Costa rican onychophoran

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dc.creatorMora López, Marielos
dc.creatorHerrera, Alvaro
dc.creatorLeón, Pedro
dc.date2015-10-23
dc.date.accessioned2016-05-03T15:31:01Z
dc.date.available2016-05-03T15:31:01Z
dc.descriptionCytologic studies of Epiperipatus biolleyi, a Costa Rican onychophoran, reveal the presence of a large genome (C-value=4.3 pg/C) with many chromosomes, and a bimodal karyotype. Analysis of maje meiosis indicates lha! all chromosomes are rod-shaped, without distinct primary constrictions. Two large bivalents are consistently associated with nucleoli. Meiosis proceeds through the pachytene stage, with a few thick bivalents that break up into many small and a few large chromosomes at the diplotene stage. At metaphase the rod-shaped bivalents seem to be pulled from the telomeres into opposite poles of the spindle, lacking well defined kinetochores. DNA isolated from E. biolleyi presents a complex profile with very low GC-content in isopycnic cesium gradients. Digestion with restriction enzymes produces a homogenous spread of molecules, except with Rae III, Hind m and Bgl n, which generate distinct bands of fluorescence. A Bgl n repeat of 1.9 Kb, has been cloned for evolutionary studies. Based on these results, many repetitive DNA familíes should be found in this genome.en-US
dc.descriptionCytologic studies of Epiperipatus biolleyi, a Costa Rican onychophoran, reveal the presence of a large genome (C-value=4.3 pg/C) with many chromosomes, and a bimodal karyotype. Analysis of maje meiosis indicates lha! all chromosomes are rod-shaped, without distinct primary constrictions. Two large bivalents are consistently associated with nucleoli. Meiosis proceeds through the pachytene stage, with a few thick bivalents that break up into many small and a few large chromosomes at the diplotene stage. At metaphase the rod-shaped bivalents seem to be pulled from the telomeres into opposite poles of the spindle, lacking well defined kinetochores. DNA isolated from E. biolleyi presents a complex profile with very low GC-content in isopycnic cesium gradients. Digestion with restriction enzymes produces a homogenous spread of molecules, except with Rae III, Hind m and Bgl n, which generate distinct bands of fluorescence. A Bgl n repeat of 1.9 Kb, has been cloned for evolutionary studies. Based on these results, many repetitive DNA familíes should be found in this genome.es-ES
dc.formatapplication/pdf
dc.identifierhttp://revistas.ucr.ac.cr/index.php/rbt/article/view/21568
dc.identifier.urihttps://hdl.handle.net/10669/27000
dc.languageeng
dc.publisherUniversidad de Costa Ricaen-US
dc.rightsacceso abierto
dc.sourceRevista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol. 44 (1) April 1996; 153-157en-US
dc.sourceRevista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol. 44 (1) April 1996; 153-157es-ES
dc.sourceRevista Biología Tropical; Vol. 44 (1) April 1996; 153-157pt-PT
dc.source2215-2075
dc.source0034-7744
dc.titleThe genome of Epiperipatus biolleyi (Peripatidae), a Costa rican onychophoranen-US
dc.typeartículo original

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Revista de Biología Tropical