A Protocol to Perform Systemic Lipopolysacharide (LPS) Challenge in Rats




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Ramírez Chan, Karol Gabriela
Quesada Yamasaki, Daniel
Fornaguera Trías, Jaime

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Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria. In animals, intraperitoneal administration of LPS, stimulates innate immunity and the production of pro-inflammatory cytokines. LPS provides an inflammatory stimulus that activates the neuroimmune and neuroendocrine systems resulting in a set of responses termed sickness behavior. The purpose of this protocol is to describe step-by-step the preparation and procedure of application of intraperitoneal injection of LPS in rats, as a guide for those researchers that want to use this assay to mount an inflammatory response. LPS intraperitoneal challenge in rats has been widely used to evaluate anti-inflammatory reagents and to address basic scientific questions.


Palabras clave

Lipopolysaccharide, LPS challenge, LPS stimulation, Endotoxin, Inflammation