Organogénesis in vitro en Dalbergia retusa (Papilonaceae)
Date
Authors
Valverde Cerdas, Lisette
Alvarado Guzmán, Laura
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Journal ISSN
Volume Title
Publisher
Universidad de Costa Rica
Abstract
Description
Se obtuvieron plantas vía Organogénesis a partir de explantes de hipocotilo de plántulas germinadas in vitro de Cocobolo (Dalbergia retusa). La inducción de los brotes adventicios se logro en un medio Murashige y Skoog (1962) que contenía cinco concentraciones de BA (benciladenina). La mejor concentración de BApara la inducción y desarrollo de los brotes fue 8,8 mM. El enrizamiento de los brotes se logro en un medio M&S (1962) a la mitad de su concentración, suplementado con 20 g/l de sacarosa y cinco concentraciones de IBA (ácido indolbutírico). El mayor número de brotes enraizados se obtuvo con 19,7 mM de IBA pero el mayor número promedio de raíces se obtuvo con 24,6 mM de IBA. Las plantas se transfirieron al invernadero para su aclimatación
Plants were obtained via organogenesis from hypocotyl explants of Dalbergia retusa from in vitro germinated seedlings. Adventitious bud induction was achieved on Murashige and Skoog medium containing five BA (benzyladenine) concentrations. The best BA concentration for budding induction and budding development was 8.8μM. Shoot rooting was obtained on half-strength modified MS basal medium, supplemented with 20g.l-1 of sucrose and five concentrations of indole-3-butyric acid (IBA). The highest number of shoot rooting was obtained with 19.7μM IBA but the highest average number of roots for plantlet was achieved with 24.6μM IBA. Plants were transferred to greenhouse conditions.
Plants were obtained via organogenesis from hypocotyl explants of Dalbergia retusa from in vitro germinated seedlings. Adventitious bud induction was achieved on Murashige and Skoog medium containing five BA (benzyladenine) concentrations. The best BA concentration for budding induction and budding development was 8.8μM. Shoot rooting was obtained on half-strength modified MS basal medium, supplemented with 20g.l-1 of sucrose and five concentrations of indole-3-butyric acid (IBA). The highest number of shoot rooting was obtained with 19.7μM IBA but the highest average number of roots for plantlet was achieved with 24.6μM IBA. Plants were transferred to greenhouse conditions.
Keywords
dalbergia retusa, micropropagation, wood species, papilonaceae, cocobolo