Cultivo in vitro de yemas axiIares de papaya (Ca- rica papaya L.). III. Efecto de concentraciones de sacarosa, ácido iodolbutírico y ácido naftalenacético sobre el enraizamiento de tallos cultivados in vitro
Archivos
Fecha
1995
Tipo
artículo original
Autores
Arrieta, Griselda
Guevara, Eric
Shancho, Guillermo
Título de la revista
ISSN de la revista
Título del volumen
Editor
Universidad de Costa Rica
Resumen
Se estudió el enraizamiento de tallos de papaya provenientes de plantas adultas y cultivados in vitro a través del efecto de concentraciones de sacarosa (1 y 2%), AlB (0,2; 0,5 mg/D, ANA(0,2; 0,5; 1,0 mg/l) y carbón activado (0,2%). El enraizamiento se realizó en dos etapas: 1/ el cultivo de los tallos sobre un medio MS con la mitad de la concen- tración de los macroelementos y conteniendo 1 o 2% de sacarosa y una auxina; y 2/ la transferencia de los tallos ocho días depués a un medio MS neutro conteniendo o no carbón activado y concentraciones variables de sacarosa. Dosis altas de auxina y en particular de ANA promovieron en general la defoliación severa de los tallos. Una concentración de 1% de sacarosa en el medio promovió un mejor enraizamiento. El tratamiento con ANA estimuló el desarrollo de callo en la base de los tallos con o sin carbón activado. El mejor enraizamiento y desarrollo de raíces se observó con el uso de AlB 0,2 mg/l y 1% de sacarosa, seguido por una transferencia a un medio MS con 1% de sacarosa y 0,2% de carbón activado.
The effect of concentrations of sucrose (1 and 2%), IBA (0.2 and 0.5 mg/D, NAA (0.2, 0.5 and 1.0 mg/l) and activated charcoal was studied on rooting of adult and in vitro cultivated papaya stalks. The rooting was conducted in two stages: 1- the cultivation of stalks on a MS medium with half the concentration of macro-elements and containing 1 or 2 % of sucrose and one auxin, and 2- the transfer of the stalks eight days later to a neutral MS medium containing, or not, activated charcoal and variable concentrations of sucrose. In general, high doses of auxins and particularly NAA caused asevere stalk defoliation. A 1 % sucrose concentration in the medium promoted a better rooting. The treatment with NAA stimulated to form a callus at the base of the stalks, with orwithout the activated charcoal. The best rooting and root growth was observed with the use of IBA 0.2 mg/l and 1 % sucrose, followed by a transfer to a MS medium with 1 % sucrose and 0.2 % of activated charcoal.
The effect of concentrations of sucrose (1 and 2%), IBA (0.2 and 0.5 mg/D, NAA (0.2, 0.5 and 1.0 mg/l) and activated charcoal was studied on rooting of adult and in vitro cultivated papaya stalks. The rooting was conducted in two stages: 1- the cultivation of stalks on a MS medium with half the concentration of macro-elements and containing 1 or 2 % of sucrose and one auxin, and 2- the transfer of the stalks eight days later to a neutral MS medium containing, or not, activated charcoal and variable concentrations of sucrose. In general, high doses of auxins and particularly NAA caused asevere stalk defoliation. A 1 % sucrose concentration in the medium promoted a better rooting. The treatment with NAA stimulated to form a callus at the base of the stalks, with orwithout the activated charcoal. The best rooting and root growth was observed with the use of IBA 0.2 mg/l and 1 % sucrose, followed by a transfer to a MS medium with 1 % sucrose and 0.2 % of activated charcoal.
Descripción
Palabras clave
AlB= ácido indolbutírico, ANA= ácido naftalenacético., Vegetative propagation, in vitro culture, buds, ea rica papaya, plant growth substances, sucrose, IBA, NAA, rooting.