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Biodegradation of sulfamethazine by Trametes versicolor: Removal from sewage sludge and identification of intermediate products by UPLC–QqTOF-MS

dc.creatorGarcía Galán, María Jesús
dc.creatorRodríguez Rodríguez, Carlos E.
dc.creatorVicent, Teresa
dc.creatorCaminal, Glòria
dc.creatorDíaz Cruz, M. Silvia
dc.creatorBarceló Cullerès, Damià
dc.date.accessioned2023-09-07T15:02:47Z
dc.date.available2023-09-07T15:02:47Z
dc.date.issued2011-11-15
dc.description.abstractDegradation of the sulfonamide sulfamethazine (SMZ) by the white-rot fungus Trametes versicolor was assessed. Elimination was achieved to nearly undetectable levels after 20 h in liquid medium when SMZ was added at 9 mg L−1 . Experiments with purified laccase and laccase-mediators resulted in almost complete removal. On the other hand, inhibition of SMZ degradation was observed when piperonilbutoxide, a cytochrome P450-inhibitor, was added to the fungal cultures. UPLC–QqTOF-MS analysis allowed the identification and confirmation of 4 different SMZ degradation intermediates produced by fungal cultures or purified laccase: desulfo-SMZ, N4 -formyl-SMZ, N4 -hydroxy-SMZ and desamino-SMZ; nonetheless SMZ mineralization was not demonstrated with the isotopically labeled sulfamethazine-phenyl-13C6 after 7 days. Inoculation of T. versicolor to sterilized sewage sludge in solid-phase systems showed complete elimination of SMZ and also of other sulfon amides (sulfapyridine, sulfathiazole) at real environmental concentrations, making this fungus an interesting candidate for further remediation research.es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro en Investigación en Contaminación Ambiental (CICA)es_ES
dc.description.sponsorshipSpanish Ministry/[project 010/PC08/3-04]//Españaes_ES
dc.description.sponsorshipSpanish Ministry of Science and Education/[CGL2007-64551]//Españaes_ES
dc.description.sponsorshipGeneralitat de Catalunya/[2009-SGR00796]//Españaes_ES
dc.identifier.citationhttps://www-sciencedirect-com.ezproxy.sibdi.ucr.ac.cr/science/article/pii/S0048969711008862es_ES
dc.identifier.doi10.1016/j.scitotenv.2011.08.022
dc.identifier.issn1879-1026
dc.identifier.issn0048-9697
dc.identifier.urihttps://hdl.handle.net/10669/89943
dc.language.isoenges_ES
dc.rightsacceso embargado
dc.sourceScience of The Total Environment, vol.409(24), pp.5505-5512.es_ES
dc.subjectSulfamethazinees_ES
dc.subjectTrametes versicolores_ES
dc.subjectSewage sludgees_ES
dc.subjectDegradation metaboliteses_ES
dc.subjectUPLC–QqTOF-MS analysises_ES
dc.titleBiodegradation of sulfamethazine by Trametes versicolor: Removal from sewage sludge and identification of intermediate products by UPLC–QqTOF-MSes_ES
dc.typeartículo originales_ES

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