Biodegradation of sulfamethazine by Trametes versicolor: Removal from sewage sludge and identification of intermediate products by UPLC–QqTOF-MS
dc.creator | García Galán, María Jesús | |
dc.creator | Rodríguez Rodríguez, Carlos E. | |
dc.creator | Vicent, Teresa | |
dc.creator | Caminal, Glòria | |
dc.creator | Díaz Cruz, M. Silvia | |
dc.creator | Barceló Cullerès, Damià | |
dc.date.accessioned | 2023-09-07T15:02:47Z | |
dc.date.available | 2023-09-07T15:02:47Z | |
dc.date.issued | 2011-11-15 | |
dc.description.abstract | Degradation of the sulfonamide sulfamethazine (SMZ) by the white-rot fungus Trametes versicolor was assessed. Elimination was achieved to nearly undetectable levels after 20 h in liquid medium when SMZ was added at 9 mg L−1 . Experiments with purified laccase and laccase-mediators resulted in almost complete removal. On the other hand, inhibition of SMZ degradation was observed when piperonilbutoxide, a cytochrome P450-inhibitor, was added to the fungal cultures. UPLC–QqTOF-MS analysis allowed the identification and confirmation of 4 different SMZ degradation intermediates produced by fungal cultures or purified laccase: desulfo-SMZ, N4 -formyl-SMZ, N4 -hydroxy-SMZ and desamino-SMZ; nonetheless SMZ mineralization was not demonstrated with the isotopically labeled sulfamethazine-phenyl-13C6 after 7 days. Inoculation of T. versicolor to sterilized sewage sludge in solid-phase systems showed complete elimination of SMZ and also of other sulfon amides (sulfapyridine, sulfathiazole) at real environmental concentrations, making this fungus an interesting candidate for further remediation research. | es_ES |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro en Investigación en Contaminación Ambiental (CICA) | es_ES |
dc.description.sponsorship | Spanish Ministry/[project 010/PC08/3-04]//España | es_ES |
dc.description.sponsorship | Spanish Ministry of Science and Education/[CGL2007-64551]//España | es_ES |
dc.description.sponsorship | Generalitat de Catalunya/[2009-SGR00796]//España | es_ES |
dc.identifier.citation | https://www-sciencedirect-com.ezproxy.sibdi.ucr.ac.cr/science/article/pii/S0048969711008862 | es_ES |
dc.identifier.doi | 10.1016/j.scitotenv.2011.08.022 | |
dc.identifier.issn | 1879-1026 | |
dc.identifier.issn | 0048-9697 | |
dc.identifier.uri | https://hdl.handle.net/10669/89943 | |
dc.language.iso | eng | es_ES |
dc.rights | acceso embargado | |
dc.source | Science of The Total Environment, vol.409(24), pp.5505-5512. | es_ES |
dc.subject | Sulfamethazine | es_ES |
dc.subject | Trametes versicolor | es_ES |
dc.subject | Sewage sludge | es_ES |
dc.subject | Degradation metabolites | es_ES |
dc.subject | UPLC–QqTOF-MS analysis | es_ES |
dc.title | Biodegradation of sulfamethazine by Trametes versicolor: Removal from sewage sludge and identification of intermediate products by UPLC–QqTOF-MS | es_ES |
dc.type | artículo original | es_ES |