Biodegradation of sulfamethazine by Trametes versicolor: Removal from sewage sludge and identification of intermediate products by UPLC–QqTOF-MS
Fecha
2011-11-15
Tipo
artículo original
Autores
García Galán, María Jesús
Rodríguez Rodríguez, Carlos E.
Vicent, Teresa
Caminal, Glòria
Díaz Cruz, M. Silvia
Barceló Cullerès, Damià
Título de la revista
ISSN de la revista
Título del volumen
Editor
Resumen
Degradation of the sulfonamide sulfamethazine (SMZ) by the white-rot fungus Trametes versicolor was assessed.
Elimination was achieved to nearly undetectable levels after 20 h in liquid medium when SMZ was added at
9 mg L−1
. Experiments with purified laccase and laccase-mediators resulted in almost complete removal. On
the other hand, inhibition of SMZ degradation was observed when piperonilbutoxide, a cytochrome
P450-inhibitor, was added to the fungal cultures. UPLC–QqTOF-MS analysis allowed the identification and
confirmation of 4 different SMZ degradation intermediates produced by fungal cultures or purified laccase:
desulfo-SMZ, N4
-formyl-SMZ, N4
-hydroxy-SMZ and desamino-SMZ; nonetheless SMZ mineralization was not
demonstrated with the isotopically labeled sulfamethazine-phenyl-13C6 after 7 days. Inoculation of T. versicolor
to sterilized sewage sludge in solid-phase systems showed complete elimination of SMZ and also of other sulfon amides (sulfapyridine, sulfathiazole) at real environmental concentrations, making this fungus an interesting
candidate for further remediation research.
Descripción
Palabras clave
Sulfamethazine, Trametes versicolor, Sewage sludge, Degradation metabolites, UPLC–QqTOF-MS analysis