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In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)

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dc.creatorValdez Melara, Marta
dc.creatorGarcía, Alexander
dc.creatorDelgado, Marlon
dc.creatorGatica Arias, Andrés Mauricio
dc.creatorRamírez Fonseca, Pilar
dc.date2015-10-06
dc.date.accessioned2016-05-03T15:29:54Z
dc.date.available2016-05-03T15:29:54Z
dc.descriptionAn efficient and reproducible method for regeneration of commercial and pure lines of tropical butternut squash (Cucurbita moschata) plants via somatic embryogenesis was developed. The influence of genotype, explant source, N6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) concentration on somatic embryogenesis induction was investigated. Friable embryogenic calli was produced from zigotic embryos (53-56%) and cotyledons from seedlings (70%) of C. moschata cv. Sello de Oro cultured on callus induction medium (CIM) supplemented with 0.5 mg/l or 3.5 mg/l 2,4-D. No embryogenic calli was obtained from leaf segments of C. moschata cv. Sello de Oro cultured on CIM supplemented with different concentrations of BAP and 2,4-D and cotyledons from seedlings of C. moschata cv. PVG 04 cultured on CIM with BAP and 2,4,5-T. Embryogenic calli induction was achieved in 75% C. moschata pure lines evaluated and calli percentage frequency range from 5% to 34%. Successful acclimatization of squash in vitro plants was achieved in the greenhouse and in the field. Regenerated plants appeared morphologically normal and set flowers and fruits with seeds that could germinate normally.en-US
dc.descriptionAn efficient and reproducible method for regeneration of commercial and pure lines of tropical butternut squash (Cucurbita moschata) plants via somatic embryogenesis was developed. The influence of genotype, explant source, N6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) concentration on somatic embryogenesis induction was investigated. Friable embryogenic calli was produced from zigotic embryos (53-56%) and cotyledons from seedlings (70%) of C. moschata cv. Sello de Oro cultured on callus induction medium (CIM) supplemented with 0.5 mg/l or 3.5 mg/l 2,4-D. No embryogenic calli was obtained from leaf segments of C. moschata cv. Sello de Oro cultured on CIM supplemented with different concentrations of BAP and 2,4-D and cotyledons from seedlings of C. moschata cv. PVG 04 cultured on CIM with BAP and 2,4,5-T. Embryogenic calli induction was achieved in 75% C. moschata pure lines evaluated and calli percentage frequency range from 5% to 34%. Successful acclimatization of squash in vitro plants was achieved in the greenhouse and in the field. Regenerated plants appeared morphologically normal and set flowers and fruits with seeds that could germinate normally.es
dc.formatapplication/pdf
dc.identifierhttp://revistas.ucr.ac.cr/index.php/rbt/article/view/21279
dc.identifier10.15517/rbt.v57i0.21279
dc.identifier.urihttps://hdl.handle.net/10669/26730
dc.languageeng
dc.publisherUniversidad de Costa Ricaen
dc.rightsacceso abierto
dc.sourceRevista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol 57 (Suplemento 1) 2009; 119-127en
dc.sourceRevista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol 57 (Suplemento 1) 2009; 119-127es
dc.sourceRevista Biología Tropical; Vol 57 (Suplemento 1) 2009; 119-127pt-PT
dc.source2215-2075
dc.source0034-7744
dc.source10.15517/rbt.v57i0
dc.subjectcucurbitsen
dc.subjectcucurbita moschataen
dc.subjectauxinsen
dc.subjectcytokininen
dc.subjectexplant sourceen
dc.subjectgenotypeen
dc.subjectCosta Ricaen
dc.subjectcucurbitses
dc.subjectcucurbita moschataes
dc.subjectauxinses
dc.subjectcytokinines
dc.subjectexplant sourcees
dc.subjectgenotypees
dc.subjectCosta Ricaes
dc.titleIn vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)en
dc.typeartículo original

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