Snake venomics of the pit vipers Porthidium nasutum, Porthidium ophryomegas, and Cerrophidion godmani from Costa Rica: Toxicological and taxonomical insights

dc.creatorLomonte, Bruno
dc.creatorRey Suárez, Paola
dc.creatorTsai, Wan-Chih
dc.creatorAngulo Ugalde, Yamileth
dc.creatorSasa Marín, Mahmood
dc.creatorGutiérrez, José María
dc.creatorCalvete Chornet, Juan José
dc.date.accessioned2017-03-24T19:29:32Z
dc.date.available2017-03-24T19:29:32Z
dc.date.issued2012-02-16
dc.description2082-02 Embargo por política editoriales_ES
dc.description.abstractWithin the Neotropical pit vipers, a lineage of primarily Middle American snake species referred to as the “Porthidium group” includes the genera Atropoides, Cerrophidion, and Porthidium. In this study, the venom proteomes of Porthidium nasutum, P. ophryomegas, and Cerrophidion godmani from Costa Rica were analyzed, and correlated to their toxic and enzymatic activities. Their HPLC profiles revealed a higher similarity between the two Porthidium species than between these and C. godmani. Proteins belonging to nine (P. nasutum), eight (P. ophryomegas), and nine (C. godmani) families were identified by mass spectrometry or N-terminal sequencing. Final cataloging of proteins and their relative abundances confirmed the close relationship between venoms of P. nasutum and P. ophryomegas, departing from that of C. godmani. Since the latter species had been taxonomically classified as Porthidium godmani previously, our venomic analyses agree with its current generic status. Venoms of P. nasutum and P. ophryomegas, despite containing abundant metalloproteinases and serine proteinases, lack procoagulant activity on human plasma, in contrast to venom of C. godmani. The latter induced strong myotoxicity in mice, which correlates with its high proportion of phospholipases A2, whereas venoms from the two Porthidium species, containing lower amounts of these enzymes, induced only mild muscle damage.es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B1-549]/UCR/Costa Ricaes_ES
dc.description.sponsorshipMinisterio de Innovación y Ciencia/[BFU2007-61563]//Españaes_ES
dc.description.sponsorshipMinisterio de Innovación y Ciencia/[BFU2010-17373]//Españaes_ES
dc.description.sponsorshipMinisterio de Ciencia y Tecnología/[108-2011]//Costa Ricaes_ES
dc.description.sponsorshipConsejo Nacional para Investigaciones Científicas y Tecnológicas/[108-2011]/CONICIT/Costa Ricaes_ES
dc.description.sponsorshipConsejo Superior de Investigaciones Científicas /[2009CR0021]/CRUSA-CSIC/Españaes_ES
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S187439191100697X
dc.identifier.doi10.1016/j.jprot.2011.12.016
dc.identifier.issn1874-3919
dc.identifier.pmid22212456
dc.identifier.urihttps://hdl.handle.net/10669/29616
dc.language.isoen_USes_ES
dc.rightsacceso embargado
dc.sourceJournal of Proteomics; Volumen 75, Número 5. 2012es_ES
dc.subjectSnake venomes_ES
dc.subjectProteomicses_ES
dc.subjectVenomicses_ES
dc.subjectMass spectrometryes_ES
dc.subjectPorthidiumes_ES
dc.subjectCerrophidiones_ES
dc.titleSnake venomics of the pit vipers Porthidium nasutum, Porthidium ophryomegas, and Cerrophidion godmani from Costa Rica: Toxicological and taxonomical insightses_ES
dc.typeartículo original

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