Snake venomics of the pit vipers Porthidium nasutum, Porthidium ophryomegas, and Cerrophidion godmani from Costa Rica: Toxicological and taxonomical insights
Fecha
2012-02-16
Tipo
artículo original
Autores
Lomonte, Bruno
Rey Suárez, Paola
Tsai, Wan-Chih
Angulo Ugalde, Yamileth
Sasa Marín, Mahmood
Gutiérrez, José María
Calvete Chornet, Juan José
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Resumen
Within the Neotropical pit vipers, a lineage of primarily Middle American snake species
referred to as the “Porthidium group” includes the genera Atropoides, Cerrophidion, and
Porthidium. In this study, the venom proteomes of Porthidium nasutum, P. ophryomegas, and
Cerrophidion godmani from Costa Rica were analyzed, and correlated to their toxic and
enzymatic activities. Their HPLC profiles revealed a higher similarity between the two
Porthidium species than between these and C. godmani. Proteins belonging to nine
(P. nasutum), eight (P. ophryomegas), and nine (C. godmani) families were identified by mass
spectrometry or N-terminal sequencing. Final cataloging of proteins and their relative
abundances confirmed the close relationship between venoms of P. nasutum and P. ophryomegas,
departing from that of C. godmani. Since the latter species had been taxonomically
classified as Porthidium godmani previously, our venomic analyses agree with its current
generic status. Venoms of P. nasutum and P. ophryomegas, despite containing abundant metalloproteinases
and serine proteinases, lack procoagulant activity on human plasma, in contrast
to venom of C. godmani. The latter induced strong myotoxicity in mice, which correlates with
its high proportion of phospholipases A2, whereas venoms from the two Porthidium species,
containing lower amounts of these enzymes, induced only mild muscle damage.
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2082-02 Embargo por política editorial
Palabras clave
Snake venom, Proteomics, Venomics, Mass spectrometry, Porthidium, Cerrophidion