Estandarización de una PCR para la detección del gen invA de Salmonella spp. en lechuga
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Authors
Chacón Jiménez, Luz María
Barrantes Jiménez, Kenia
García, Cristina
Achí Araya, María Rosario
Journal Title
Journal ISSN
Volume Title
Publisher
Revista de la Sociedad Venezolana de Microbiología 2010; 30(1): 18-23
Abstract
Salmonella spp. es un patógeno bacteriano muy importante causante de diarreas, que es transmitido tanto por la vía fecal-oral,
como por alimentos y agua contaminados. En este trabajo se estandarizó una técnica de PCR en lechuga para la detección del gen invA de
Salmonella spp.; dicho gen se relaciona con el proceso de invasión al epitelio intestinal. Con la PCR desarrollada en este trabajo se logró
estandarizar un método que permite la amplificación del gen invA con una detección de 102 UFC/25 g. Este método acorta los tiempos de
respuesta de los resultados presuntivos y brinda información complementaria al cultivo tradicional del patógeno. El estudio del gen invA
establece el potencial patógeno del microorganismo presente en la muestra, lo que puede ser de utilidad para la salud pública.
Salmonella spp. is a very important bacterial pathogen that causes diarrhea and which is transmitted both through the fecal-oral pathway, as by contaminated food and water. In this study we standardized a PCR method in lettuce for the detection of the Salmonella spp. invA gene. This gene is related to the invasion of the intestinal epithelium process. With the PCR method developed in this study we were able to standardize a method which permits the amplification of the invA gene with a 102 CFU/25 g detection. This method shortens the response times of the presumptive results and gives complementary information to the traditional culture of the pathogen. The study of the invA gene establishes the pathogenic potential of the microorganism present in the sample, which can be useful for public health purposes.
Salmonella spp. is a very important bacterial pathogen that causes diarrhea and which is transmitted both through the fecal-oral pathway, as by contaminated food and water. In this study we standardized a PCR method in lettuce for the detection of the Salmonella spp. invA gene. This gene is related to the invasion of the intestinal epithelium process. With the PCR method developed in this study we were able to standardize a method which permits the amplification of the invA gene with a 102 CFU/25 g detection. This method shortens the response times of the presumptive results and gives complementary information to the traditional culture of the pathogen. The study of the invA gene establishes the pathogenic potential of the microorganism present in the sample, which can be useful for public health purposes.
Description
artículo (arbitrado) -- Universidad de Costa Rica. Instituto de Investigaciones en Salud, 2010
Keywords
Salmonella, Bacteria, Reacción en Cadena de la Polimerasa, Sativa, Salud pública