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A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-l-Ascorbic Acid Trisodium Salt as a Substrate
dc.creator | Alvarado Gámez, Ana Lorena | |
dc.creator | Alonso Lomillo, María Asunción | |
dc.creator | Domínguez Renedo, Olga | |
dc.creator | Arcos Martínez, María Julia | |
dc.date.accessioned | 2020-02-28T15:48:24Z | |
dc.date.available | 2020-02-28T15:48:24Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | https://www.mdpi.com/1424-8220/15/2/2232 | |
dc.identifier.issn | 1424-8220 | |
dc.identifier.uri | https://hdl.handle.net/10669/80662 | |
dc.description.abstract | This paper presents a chronoamperometric method to determine tungsten in water using screen-printed carbon electrodes modified with gold nanoparticles and cross linked alkaline phosphatase immobilized in the working electrode. Enzymatic activity over 2-phospho-l-ascorbic acid trisodium salt, used as substrate, was affected by tungsten ions, which resulted in a decrease of chronoamperometric current, when a potential of 200 mV was applied on 10 mM of substrate in a Tris HCl buffer pH 8.00 and 0.36 M of KCl. Calibration curves for the electrochemical method validation, give a reproducibility of 5.2% (n = 3), a repeatability of 9.4% (n = 3) and a detection limit of 0.29 ± 0.01 μM. Enriched tap water, purified laboratory water and bottled drinking water, with a certified tungsten reference solution traceable to NIST, gave a recovery of 97.1%, 99.1% and 99.1% respectively (n = 4 in each case) and a dynamic range from 0.6 to 30 μM. This study was performed by means of a Lineweaver–Burk plot, showing a mixed kinetic inhibition. | es_ES |
dc.description.sponsorship | Universidad de Costa Rica/[804-B2-297]/UCR/Costa Rica | es_ES |
dc.description.sponsorship | Junta de Castilla y León/[BU212A12-2]//España | es_ES |
dc.description.sponsorship | Ministerio de Ciencia e Innovación/[TEC2009-12029]/MICINN/España | es_ES |
dc.description.sponsorship | Fondo Europeo de Desarrollo Regional/[INNPACTO SERIBIO 2011-2014]/FEDER/España | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Sensors, vol.15(2), pp.2232-2243 | es_ES |
dc.subject | Alkaline phosphatase | es_ES |
dc.subject | Chronoamperometry | es_ES |
dc.subject | 2-phospho-l-ascorbic acid trisodium salt | es_ES |
dc.subject | Screen printed carbon electrode | es_ES |
dc.subject | Tungsten | es_ES |
dc.title | A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-l-Ascorbic Acid Trisodium Salt as a Substrate | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.3390/s150202232 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Electroquímica y Energía Química (CELEQ) | es_ES |
dc.description.procedence | UCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Química | es_ES |
dc.identifier.codproyecto | 804-B2-297 | |
dc.identifier.pmid | PMC4367303 | |
dc.identifier.pmid | 25621602 |
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