Listar Microbiología por autor "07062725-ae52-4388-80a7-a07d86f6d36d"
Mostrando ítems 1-20 de 23
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An interactive database for the investigation of high-density peptide microarray guided interaction patterns and antivenom cross-reactivity
Krause, Kamille E.; Jenkins, Timothy P.; Skaarup, Carina; Engmark, Mikael Mikael Gerling; Casewell, Nicholas R.; Ainsworth, Stuart; Lomonte, Bruno; Fernández Ulate, Julián; Gutiérrez, José María; Lund, Ole; Laustsen, Andreas Hougaard (2020)Snakebite envenoming is a major neglected tropical disease that affects millions of people every year. The only effective treatment against snakebite envenoming consists of unspecified cocktails of polyclonal antibodies ... -
Antibody-dependent enhancement of toxicity of myotoxin II from Bothrops asper
Sørensen, Christoffer V.; Fernández, Julián; Adams, Anna Christina; Wildenauer, Helen H. K.; Schoffelen, Sanne; Ledsgaard, Line; Pucca, Manuela Berto; Fiebig, Michael; Cerni, Felipe A.; Tulika, Tulika; Voldborg, Bjørn; Karatt Vellatt, Aneesh; Morth, J. Preben; Ljungars, Anne; Grav, Lise M.; Lomonte, Bruno; Laustsen, Andreas Hougaard (2024)Improved therapies are needed against snakebite envenoming, which kills and permanently disables thousands of people each year. Recently developed neutralizing monoclonal antibodies against several snake toxins have ... -
Cross-recognition of a pit viper (Crotalinae) polyspecific antivenom explored through high-density peptide microarray epitope mapping
Engmark, Mikael Gerling; Lomonte, Bruno; Gutiérrez, José María; Laustsen, Andreas Hougaard; de Masi, Federico; Andersen, Mikael Rørdam; Lund, Ole (2017-07)Snakebite antivenom is a 120 years old invention based on polyclonal mixtures of antibodies purified from the blood of hyper-immunized animals. Knowledge on antibody recognition sites (epitopes) on snake venom proteins ... -
Danger in the reef: Proteome, toxicity, and neutralization of the venom of the olive sea snake, Aipysurus laevis
Laustsen, Andreas Hougaard; Gutiérrez, José María; Rasmussen, Arne R.; Engmark, Mikael Gerling; Gravlund, Peter; Sanders, Kate L.; Lohse, Brian; Lomonte, Bruno (2015-12-01)Four specimens of the olive sea snake, Aipysurus laevis, were collected off the coast of Western Australia, and the venom proteome was characterized and quantitatively estimated by RP-HPLC, SDS-PAGE, and MALDI-TOF-TOF ... -
Discovery and optimization of a broadly-neutralizing human antibody against long-chain α-neurotoxins from snakes
Ledsgaard, Line; Wade, Jack; Jenkins, Timothy P.; Boddum, Kim; Oganesyan, Irina; Harrison, Julian A.; Villar, Pedro; Leah, Rachael A.; Zenobi, Renato; Schoffelen, Sanne; Voldborg, Bjørn; Ljungars, Anne; McCafferty, John; Lomonte, Bruno; Gutiérrez, José María; Laustsen, Andreas Hougaard; Karatt Vellatt, Aneesh (2023-02-08)Snakebite envenoming continues to claim many lives across the globe, necessitating the development of improved therapies. To this end, broadly-neutralizing human monoclonal antibodies may possess advantages over current ... -
Editorial: Novel immunotherapies against envenomings by snakes and other venomous animals
Laustsen, Andreas Hougaard; Ainsworth, Stuart; Lomonte, Bruno; Kini, R. Manjunatha; Chávez Olórtegui, Carlos (2020)Editorial on the Research Topic -
Exploration of immunoglobulin transcriptomes from mice immunized with three-finger toxins and phospholipases A2 from the Central American coral snake, Micrurus nigrocinctus
Laustsen, Andreas Hougaard; Engmark, Mikael Gerling; Clouser, Christopher; Timberlake, Sonia; Vigneault, Francois; Gutiérrez, José María; Lomonte, Bruno (2017)Snakebite envenomings represent a neglected public health issue in many parts of the rural tropical world. Animal-derived antivenoms have existed for more than a hundred years and are effective in neutralizing snake venom ... -
Exploring the venom of the forest cobra snake: Toxicovenomics and antivenom profiling of Naja melanoleuca
Lauridsen, Line Præst; Laustsen, Andreas Hougaard; Lomonte, Bruno; Gutiérrez, José María (2017-01-06)A toxicovenomic analysis of the venomof the forest cobra, N. melanoleuca,was performed, revealing the presence of a total of 52 proteins by proteomics analysis. The most abundant proteins belong to the three-finger ... -
From Fangs to Pharmacology: The Future of Snakebite Envenoming Therapy
Laustsen, Andreas Hougaard; Engmark, Mikael Gerling; Milbo, Christina; Johannesen, Jonás; Lomonte, Bruno; Gutiérrez, José María; Lohse, Brian (2016)The snake is the symbol of medicine due to its association with Asclepius, the Greek God of medicine, and so with good reasons. More than 725 species of venomous snakes have toxins specifically evolved to exert potent ... -
High-density peptide microarray exploration of the antibody response in a rabbit immunized with a neurotoxic venom fraction
Engmark, Mikael Gerling; Jespersen, Martin Closter; Lomonte, Bruno; Lund, Ole; Laustsen, Andreas Hougaard (2017-11)Polyvalent snakebite antivenoms derive their therapeutic success from the ability of their antibodies to neutralize venom toxins across multiple snake species. This ability results from a production process involving ... -
High-throughput immuno-profiling of mamba (Dendroaspis) venom toxin epitopes using high-density peptide microarrays
Engmark, Mikael Gerling; Andersen, Mikael Rørdam; Laustsen, Andreas Hougaard; Patel, Jigar; Sullivan, Eric; de Masi, Federico; Hansen, Christian S.; Kringelum, Jens V.; Lomonte, Bruno; Gutiérrez, José María; Lund, Ole (2016-11-08)Snakebite envenoming is a serious condition requiring medical attention and administration of antivenom. Current antivenoms are antibody preparations obtained from the plasma of animals immunised with whole venom(s) and ... -
In vitro discovery of a human monoclonal antibody that neutralizes lethality of cobra snake venom.
Ledsgaard, Line; Laustsen, Andreas Hougaard; Pus, Urska; Wade, Juli S.; Villar, Pedro; Boddum, Kim; Slanvy, Peter; Masters, Edward W.; Arias Oviedo, Ana Silvia; Oscoz, Saioa; Griffiths, Daniel T.; Luther, Alice M.; Linholm, Majken; Leah, Rachael A.; Møller, Marie Sofie; Ali, Hanif; McCafferty, John; Lomonte, Bruno; Gutiérrez, José María; Karatt Vellatt, Aneesh (2022)The monocled cobra (Naja kaouthia) is among the most feared snakes in Southeast Asia due to its toxicity, which is predominantly derived from long-chain α-neurotoxins. The only specific treatment for snakebite envenoming ... -
In vivo neutralization of Myotoxin II, a phospholipase A2 homolog from Bothrops asper venom, using peptides discovered via phage display technology
Laustsen, Andreas Hougaard; Gless, Bengt H.; Jenkins, Timothy P.; Meyhoff Madsen, Maria; Bjärtun, Johanna; Munk, Andreas S.; Oscoz, Saioa; Fernández Ulate, Julián; Gutiérrez, José María; Lomonte, Bruno; Lohse, Brian (2022)Many snake venom toxins cause local tissue damage in prey and victims, which constitutes an important pathology that is challenging to treat with existing antivenoms. One of the notorious toxins that causes such effects ... -
In vivo neutralization of the dendrotoxin-mediated neurotoxicity of black mamba venom by oligoclonal human IgG monoclonal antibodies
Laustsen, Andreas Hougaard; Karatt Vellatt, Aneesh; Masters, Edward W.; Arias Oviedo, Ana Silvia; Pus, Urska; Knudsen, Cecilie; Oscoz, Saioa; Slavny, Peter; Griffiths, Daniel T.; Luther, Alice M.; Leah, Rachael A.; Lindholm, Majken; Lomonte, Bruno; Gutiérrez, José María; McCafferty, John (2018)The black mamba (Dendroaspis polylepis) is one of the most feared snake species of the African savanna. It has a potent, fast-acting neurotoxic venom comprised of dendrotoxins and α-neurotoxins associated with high ... -
Myotoxin-neutralizing peptides
Lohse, Brian; Laustsen, Andreas Hougaard; Fernández Ulate, Julián; Cob, Saioa; Lomonte, Bruno; Gutiérrez, José María (2018-03-27)The present invention relates to polypeptides having myotoxin-neutralizing properties and their use for treatment of envenomation. The present invention further relates to methods for neutralizing a venom using the polypeptide ... -
Novel snakebite therapeutics must be tested in appropriate rescue models to robustly assess their preclinical efficacy
Knudsen, Cecilie; Casewell, Nicholas R.; Lomonte, Bruno; Gutiérrez, José María; Vaiyapuri, Sakthivel; Laustsen, Andreas Hougaard (2020)In the field of antivenom research, development, and manufacture, it is often advised to follow the World Health Organization’s (WHO) guidelines for the production, control, and regulation of snake antivenom immunoglobulins, ... -
Pitfalls to avoid when using phage display for snake toxins
Laustsen, Andreas Hougaard; Lauridsen, Line Præst; Lomonte, Bruno; Andersen, Mikael Rørdam; Lohse, Brian (2017-02)Antivenoms against bites and stings from snakes, spiders, and scorpions are associated with immunological side effects and high cost of production, since these therapies are still derived from the serum of hyper-immunized ... -
Pros and cons of different therapeutic antibody formats for recombinant antivenom development
Laustsen, Andreas Hougaard; Gutiérrez, José María; Knudsen, Cecilie; Johansen, Kristoffer H.; Bermúdez Méndez, Erick; Cerni, Felipe A.; Jürgensen, Jonas A.; Ledsgaard, Line; Martos Esteban, Andrea; Øhlenschlæger, Mia; Pus, Urska; Andersen, Mikael Rørdam; Lomonte, Bruno; Engmark, Mikael Gerling; Pucca, Manuela Berto (2018-05)Antibody technologies are being increasingly applied in the field of toxinology. Fuelled by the many advances in immunology, synthetic biology, and antibody research, different approaches and antibody formats are being ... -
Selecting key toxins for focused development of elapid snake antivenoms and inhibitors guided by a Toxicity Score
Laustsen, Andreas Hougaard; Lohse, Brian; Lomonte, Bruno; Engmark, Mikael Gerling; Gutiérrez, José María (2015-09-15)For more than 100 years, antivenoms have been produced by traditional methods of immunization of large mammals with mixtures of snake venoms (World Health Organization, 2010 and Gutiérrez et al., 2011). With the introduction ... -
Snake venomics of monocled cobra (Naja kaouthia) and investigation of human IgG response against venom toxins
Laustsen, Andreas Hougaard; Gutiérrez, José María; Lohse, Brian; Rasmussen, Arne R.; Fernández Ulate, Julián; Milbo, Christina; Lomonte, Bruno (2015-06)The venom proteome of the monocled cobra, Naja kaouthia, from Thailand, was characterized by RPHPLC, SDS-PAGE, and MALDI-TOF-TOF analyses, yielding 38 different proteins that were either identified or assigned to families. ...