Pitfalls to avoid when using phage display for snake toxins
artículo original
Fecha
2017-02Autor
Laustsen, Andreas Hougaard
Lauridsen, Line Præst
Lomonte, Bruno
Andersen, Mikael Rørdam
Lohse, Brian
Metadatos
Mostrar el registro completo del ítemResumen
Antivenoms against bites and stings from snakes, spiders, and scorpions are associated with immunological side effects and high cost of production, since these therapies are still derived from the serum of hyper-immunized production animals. Biotechnological innovations within envenoming therapies are thus warranted, and phage display technology may be a promising avenue for bringing antivenoms into the modern era of biologics. Although phage display technology represents a robust
and high-throughput approach for the discovery of antibody-based antitoxins, several pitfalls may present themselves when animal toxins are used as targets for phage display selection. Here, we report selected critical challenges from our own phage display experiments associated with biotinylation of antigens, clone picking, and the presence of amber codons within antibody fragment structures in some phage display libraries. These challenges may be detrimental to the outcome of
phage display experiments, and we aim to help other researchers avoiding these pitfalls by presenting their solutions.
External link to the item
10.1016/j.toxicon.2016.12.010Colecciones
- Microbiología [1171]