dc.creator | Alpízar Alpízar, Warner | |
dc.creator | Didrik Laerum, Ole | |
dc.creator | Christensen, Ib J. | |
dc.creator | Ovrebo, Kjell | |
dc.creator | Skarstein, Arne | |
dc.creator | Høyer Hansen, Gunilla | |
dc.creator | Ploug, Michael | |
dc.creator | Illemann, Martin | |
dc.date.accessioned | 2017-12-21T20:17:27Z | |
dc.date.available | 2017-12-21T20:17:27Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | http://journals.sagepub.com/doi/10.1369/0022155416656173 | |
dc.identifier.issn | 0022-1554 | |
dc.identifier.issn | 1551-5044 | |
dc.identifier.uri | https://hdl.handle.net/10669/73731 | |
dc.description.abstract | The tissue inhibitor of metalloproteinase-1 (TIMP-1) inhibits the extracellular matrix–degrading activity of several matrix metalloproteinases, thereby regulating cancer cell invasion and metastasis. Studies describing the expression pattern and cellular localization of TIMP-1 in gastric cancer are, however, highly discordant. We addressed these inconsistencies by performing immunohistochemistry and in situ hybridization analyses in a set of 49 gastric cancer lesions to reexamine the TIMP-1 localization. In addition, we correlated these findings to clinicopathological parameters. We show that strong expression of TIMP-1 protein and mRNA was observed in a subpopulation of stromal fibroblast-like cells at the periphery of the cancer lesions. In a few cases, a small fraction of cancer cells showed weak expression of TIMP-1 protein and mRNA. The stromal TIMP-1-expressing cells were mainly tumor-associated myofibroblasts. In the normal-appearing mucosa, scattered TIMP-1 protein was only found in chromogranin A positive cells. TIMP-1-positive myofibroblasts at the invasive front of the tumors were more frequently seen in intestinal than in diffuse histological subtype cases (p=0.009). A significant trend to a higher number of cases showing TIMP-1 staining in myofibroblasts with increasing tumor, node, metastasis (TNM) stage was also revealed (p=0.041). In conclusion, tumor-associated myofibroblasts are the main source of increased TIMP-1 expression in gastric cancer. | es_ES |
dc.description.sponsorship | Danish Cancer Society/[R2-A261-09-S2]//Europa | es_ES |
dc.description.sponsorship | European Community’s Seventh Framework Program/[201279]/FP7/Europa | es_ES |
dc.description.sponsorship | Axel Muusfeldts Fond/[]//Europa | es_ES |
dc.description.sponsorship | Krista og Viggo Petersens Fond/[]/KV/Europa | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Journal of Histochemistry & Cytochemistry; Volumen 64, Número 8, 2016 | es_ES |
dc.subject | Gastric cancer | es_ES |
dc.subject | Immunohistochemistry | es_ES |
dc.subject | In situ hybridization | es_ES |
dc.subject | Invasion | es_ES |
dc.subject | Myofibroblasts | es_ES |
dc.subject | Neuroendocrine cells | es_ES |
dc.subject | TIMP-1 | es_ES |
dc.title | Tissue Inhibitor of Metalloproteinase-1 Is Confined to Tumor-Associated Myofibroblasts and Is Increased With Progression in Gastric Adenocarcinoma | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1369/0022155416656173 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Estructuras Microscópicas (CIEMIC) | es_ES |
dc.identifier.pmid | 27370797 | |