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Pathological alterations induced by neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi snake venom
(2001-06)
The pathological alterations induced by neuwiedase, a 22 kDa class P-I metalloproteinase from the venom of the South American pit viper Bothrops neuwiedi, were studied in mice. Neuwiedase was devoid of hemorrhagic activity ...
Caprylic acid fractionation of hyperimmune horse plasma: description of a simple procedure for antivenom production
(1994)
A simple methodology for hyperimmune horse plasma fractionation, based on caprylic acid precipitation, is described. Optimal conditions for fractionation were studied; the method gives best results when concentrated caprylic ...
Exploring the venom of the forest cobra snake: Toxicovenomics and antivenom profiling of Naja melanoleuca
(2017-01-06)
A toxicovenomic analysis of the venomof the forest cobra, N. melanoleuca,was performed, revealing the presence
of a total of 52 proteins by proteomics analysis. The most abundant proteins belong to the three-finger ...
An alternative in vitro method for testing the potency of the polyvalent antivenom produced in Costa Rica
(1988)
The ability of several batches of polyvalent antivenom to neutralize indirect hemolytic activity of Bothrops asper venom was studied using a sensitive plate test. All samples of antivenom tested effectively neutralized ...
Structural and functional characterization of myotoxin I, a Lys49 phospholipase A(2) homologue from Bothrops moojeni (Caissaca) snake venom
(2000-01-01)
Myotoxin-I (MjTX-I) was purified to homogeneity from the venom of Bothrops moojeni by ion-exchange chromatography on CM-Sepharose. Its molecular weight, estimated by SDS–PAGE, was 13,400 (reduced) or 26,000 (unreduced). ...
Inhibition of toxic activities of Bothrops asper venom and other crotalid snake venoms by a novel neutralizing mixture
(1997-12)
The majority of snake bites in Central America are caused by Bothrops asper, whose venom induce complex local effects such as myonecrosis, edema and especially hemorrhage. These effects are only partially neutralized by ...
Immunochemical characterization of Micrurus nigrocinctus nigrocinctus venom with monoclonal and polyclonal antibodies
(1994)
Eleven murine monocloncal antibodies (MAbs) against Micrurus nigrocinctus nigrocinctus venom were produced and partially characterized. When M. n. nigrocinctus venom proteins were separated by SDS-PAGE under non-reducing ...
Detection of proteins antigenically-related to Bothrops asper myotoxin in crotaline snake venoms
(1987)
The presence of components antigenically related to Bothrops asper myotoxin was investigated by Western blotting and immunoelectrophoretic techniques. B. asper myotoxin is a non-glycosylated monomeric phospholipase A with ...
Phospholipases A2: Unveiling the secrets of a functionally versatile group of snake venom toxins
(2013-02)
Phospholipases A2 (PLA2s) are abundant components of snake venoms, where they play toxic and digestive roles. Despite having a similar three-dimensional structure, venom PLA2s exert an amazing variety of toxic and ...
Assessing endotoxins in equine-derived snake antivenoms: Comparison of the USP pyrogen test and the Limulus Amoebocyte Lysate assay (LAL)
(2015-08-29)
Snake antivenoms are parenterally administered; therefore, endotoxin content must be strictly controlled. Following international indications to calculate endotoxin limits, it was determined that
antivenom doses between ...