Production of selenium nanoparticles occurs through an interconnected pathway of sulphur metabolism and oxidative stress response in Pseudomonas putida KT2440

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dc.creatorAvendaño Vega, Roberto
dc.creatorMuñoz Montero, Said
dc.creatorRojas Gätjens, Diego
dc.creatorFuentes Schweizer, Paola
dc.creatorVieto Fonseca, Sofía
dc.creatorMontenegro, Rafael
dc.creatorSalvador, Manuel
dc.creatorFrew, Rufus
dc.creatorKim, Juhyun
dc.creatorChavarría Vargas, Max
dc.creatorJiménez Ramírez, José Ignacio
dc.date.accessioned2024-06-24T14:14:52Z
dc.date.available2024-06-24T14:14:52Z
dc.date.issued2023-01-22
dc.description.abstractThe soil bacterium Pseudomonas putida KT2440 has been shown to produce selenium nanoparticles aerobically from selenite; however, the molecular actors involved in this process are unknown. Here, through a combination of genetic and analytical techniques, we report the first insights into selenite metabolism in this bacterium. Our results suggest that the reduction of selenite occurs through an interconnected metabolic network involving central metabolic reactions, sulphur metabolism, and the response to oxidative stress. Genes such as sucA, D2HGDH and PP_3148 revealed that the 2-ketoglutarate and glutamate metabolism is important to convert selenite into selenium. On the other hand, mutations affecting the activity of the sulphite reductase decreased the bacteria's ability to transform selenite. Other genes related to sulphur metabolism (ssuEF, sfnCE, sqrR, sqr and pdo2) and stress response (gqr, lsfA, ahpCF and sadI) were also identified as involved in selenite transformation. Interestingly, suppression of genes sqrR, sqr and pdo2 resulted in the production of selenium nanoparticles at a higher rate than the wild-type strain, which is of biotechnological interest. The data provided in this study brings us closer to understanding the metabolism of selenium in bacteria and offers new targets for the development of biotechnological tools for the production of selenium nanoparticles.es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones en Productos Naturales (CIPRONA)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Electroquímica y Energía Química (CELEQ)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Químicaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[809-B5-A68]/UCR/Costa Ricaes_ES
dc.description.sponsorshipBiology and Biotechnology Research Council/[BB/T011289/1]/BBSRC/Reino Unidoes_ES
dc.description.sponsorshipEuropean Community's H2020 Programme/[101000794]//Reino Unidoes_ES
dc.description.sponsorshipHorizon Europe: BIOremediation systems exploiting SYnergieS for improved removal of Mixed pOllutants/[101060211]/BIOSYSMO/Reino Unidoes_ES
dc.description.sponsorshipHorizon Europe: BIOremediation systems exploiting SYnergieS for improved removal of Mixed pOllutants/[10045495]/BIOSYSMO/Reino Unidoes_ES
dc.description.sponsorshipEuropean Community's H2020 Programme/[FNR-11-2020]//Reino Unidoes_ES
dc.identifier.citationhttps://enviromicro-journals.onlinelibrary.wiley.com/doi/full/10.1111/1751-7915.14215es_ES
dc.identifier.codproyecto809-B5-A68
dc.identifier.doi10.1111/1751-7915.14215
dc.identifier.issn1751-7915
dc.identifier.urihttps://hdl.handle.net/10669/91597
dc.language.isoenges_ES
dc.rightsacceso abiertoes_ES
dc.sourceMicrobial Biotechnology, 16(5), 931-946es_ES
dc.subjectseleniumes_ES
dc.subjectPseudomonas putida KT2440es_ES
dc.subjectsulphur metabolismes_ES
dc.subjectoxidative stress responsees_ES
dc.titleProduction of selenium nanoparticles occurs through an interconnected pathway of sulphur metabolism and oxidative stress response in Pseudomonas putida KT2440es_ES
dc.typeartículo originales_ES

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