A novel seed baiting technique for the epiphytic orchid Rhynchostele cervantesii, a means to acquire mycorrhizal fungi from protocorms
Date
Authors
Cruz Higareda, Jesús Bernardo
Luna Rosales, Bárbara Susana
Barba Álvarez, Amadeo
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad de Costa Rica
Abstract
Description
We developed a new and novel seed baiting technique sowing mature seeds of the epiphyitic orchid Rhynchostele cervantesii under natural conditions, We introduced a sponge in each package that may serve as a reservoir for water retention to benefit germination; In three of 22 packets we found protocorms in an early stage of development, six of wich were of sufficient size to warrant fungal isolations; Nine strains were isolated in pure culture and were inoculated on seeds and protocorms under in vitro conditions.
We developed a new and novel seed baiting technique sowing mature seeds of the epiphyitic orchid Rhynchostele cervantesii under natural conditions, We introduced a sponge in each package that may serve as a reservoir for water retention to benefit germination; In three of 22 packets we found protocorms in an early stage of development, six of wich were of sufficient size to warrant fungal isolations; Nine strains were isolated in pure culture and were inoculated on seeds and protocorms under in vitro conditions.
We developed a new and novel seed baiting technique sowing mature seeds of the epiphyitic orchid Rhynchostele cervantesii under natural conditions, We introduced a sponge in each package that may serve as a reservoir for water retention to benefit germination; In three of 22 packets we found protocorms in an early stage of development, six of wich were of sufficient size to warrant fungal isolations; Nine strains were isolated in pure culture and were inoculated on seeds and protocorms under in vitro conditions.
Keywords
Orchid, Epiphytic, Seed, Baiting, Mycorrhiza, Rhynchostele, Orchid, Epiphytic, Seed, Baiting, Mycorrhiza, Rhynchostele