Microplastics in feed cause sublethal changes in the intestinal microbiota and a non-specific immune response indicator of the freshwater crayfish Procambarus clarkii (Decapoda: Cambaridae)

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dc.creatorGuillén Watson, Rossy
dc.creatorArias Andrés, María de Jesús
dc.creatorRojas Jiménez, Keilor Osvaldo
dc.creatorWehrtmann, Ingo S.
dc.date.accessioned2023-08-18T16:06:42Z
dc.date.available2023-08-18T16:06:42Z
dc.date.issued2023-07-18
dc.description.abstractMicroplastics (MP) are a hazardous pollutant of global concern that threatens aquatic ecosystems and public health. We used the invasive, cosmopolitan, and environmentally versatile red swamp crayfish Procambarus clarkii as a model to study the effects of MP on the intestinal microbiome. Crayfish collected from the environment were compared with specimens exposed to recycled Polyethylene terephthalate (rPET) MP in feed (30%) for 96 h in the laboratory and a control group. We analyzed the 16S rRNA of the intestinal bacteria by PCR-DGGE and high-throughput sequencing. MP exposure caused dysbiosis of the intestinal microbiota, with an increase in Alphaproteobacteria and Actinobacteria. We detected higher abundance of opportunistic genera such as Klebsiella, Acinetobacter, Hydromonas, Pseudomonas, Gemmobacter, and Enterobacter on MP fed organisms. Moreover, MP exposure reduced the abundance of Clostridia and Bateroidetes, which are important for immune system development and pathogen prevention. Furthermore, MP exposure decreased the phenoloxidase (PO) immune response in crayfish. There was a significant difference in the richness of intestinal bacterial communities after consumption of food contaminated with MP, likely increasing the abundance of opportunistic bacteria in the intestinal microbiota. Our results suggest that MP alter the gut microbial composition and impair the health of P. clarkii.es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologíaes_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Ciencias del Mar y Limnología (CIMAR)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biodiversidad y Ecología Tropical (CIBET)es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[808-C0-656]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[808-B8-297]/UCR/Costa Ricaes_ES
dc.identifier.citationhttps://www.frontiersin.org/articles/10.3389/fmicb.2023.1197312/fulles_ES
dc.identifier.codproyecto808-C0-656
dc.identifier.codproyecto808-B8-297
dc.identifier.doi10.3389/fmicb.2023.1197312
dc.identifier.issn2332-8266
dc.identifier.issn2371-9818
dc.identifier.urihttps://hdl.handle.net/10669/89900
dc.language.isoenges_ES
dc.rightsacceso abierto
dc.sourceFrontiers in Microbiology, Vol.14, pp. 1-13es_ES
dc.subjectGut microbiotaes_ES
dc.subjectCrayfishes_ES
dc.subjectMicroplasticses_ES
dc.subjectFRESHWATERes_ES
dc.subjectDysbiosises_ES
dc.titleMicroplastics in feed cause sublethal changes in the intestinal microbiota and a non-specific immune response indicator of the freshwater crayfish Procambarus clarkii (Decapoda: Cambaridae)es_ES
dc.typeartículo originales_ES

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