Protocol for the preparation of Arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization

Bolaños Villegas, Pablo Alberto; Yang, Xiaohui; Makaroff, Christopher A.; Jauh, Guang-Yuh

Protocol for the preparation of Arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization

dc.creator	Bolaños Villegas, Pablo Alberto
dc.creator	Yang, Xiaohui
dc.creator	Makaroff, Christopher A.
dc.creator	Jauh, Guang-Yuh
dc.date.accessioned	2015-02-24T21:43:55Z
dc.date.available	2015-02-24T21:43:55Z
dc.date.issued	2014-04-05
dc.description	artículo -- Universidad de Costa Rica. Escuela de Agronomía, 2014	es_ES
dc.description.abstract	This protocol is a more detailed version of previous protocols (Yang et al., 2011; Bolaños-Villegas et al., 2013) developed for the examination of meiotic chromosome spreads. Meiotic chromosome spreads are useful to determine the presence of defects in chromosome pairing and segregation. The protocol also describes how to perform fluorescent in situ hybridization experiments with a centromere probe used to label chromosomes.	es_ES
dc.description.procedence	UCR::Vicerrectoría de Docencia::Ciencias Agroalimentarias::Facultad de Ciencias Agroalimentarias::Escuela de Agronomía	es_ES
dc.description.sponsorship	This work was supported by research grants from Academia Sinica (Taiwan), the National Science and Technology Program for Agricultural Biotechnology (NSTP/AB, 098S0030055-AA, Taiwan) and the National Science Council (99-2321-B-001-036-MY3) to G-YJ, by a grant (MCB0718191) to CAM from the National Science Foundation (NSF) and by a study abroad contract to PBV from the University of Costa Rica.	es_ES
dc.identifier.citation	http://www.bio-protocol.org/e1102
dc.identifier.uri	https://hdl.handle.net/10669/11313
dc.language.iso	en_US	es_ES
dc.publisher	Bio-protocol 4(7), e1102, 2014	es_ES
dc.rights	acceso abierto
dc.subject	Meiosis	es_ES
dc.subject	Arabidopsis thaliana	es_ES
dc.subject	Cromosomas	es_ES
dc.subject	Hibridización in situ fluorescente	es_ES
dc.title	Protocol for the preparation of Arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization	es_ES
dc.type	artículo original

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