Stable production of recombinant SARS-CoV-2 receptor-binding domain in mammalian cells with co-expression of a fluorescent reporter and its validation as antigenic target for COVID-19 serology testing

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dc.creatorCorrales Aguilar, Eugenia
dc.creatorArias Arias, Jorge Luis
dc.creatorMolina Castro, Silvia Elena
dc.creatorMonturiol Gross, Laura
dc.creatorLomonte, Bruno
dc.date.accessioned2023-02-07T20:05:43Z
dc.date.available2023-02-07T20:05:43Z
dc.date.issued2023-01
dc.description.abstractSARS-CoV-2 receptor binding domain (RBD) recognizes the angiotensin converting enzyme 2 (ACE2) receptor in host cells that enables infection. Due to its antigenic specificity, RBD production is important for development of serological assays. Here we have established a system for stable RBD production in HEK 293T mammalian cells that simultaneously express the recombinant fluorescent protein dTomato, which enables kinetic monitoring of RBD expression by fluorescence microscopy. In addition, we have validated the use of this recombinant RBD in an ELISA assay for the detection of anti-RBD antibodies in serum samples of COVID-19 convalescent patients. Recombinant RBD generated using this approach can be useful for generation of antibody-based therapeutics against SARS-CoV-2, as well serological assays aimed to test antibody responses to this relevant virus.es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-C1-015]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[803-C0-344]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[803-C1-509]/UCR/Costa Ricaes_ES
dc.identifier.citationhttps://doi.org/10.1016/j.btre.2022.e00780es_ES
dc.identifier.codproyecto741-C1-015
dc.identifier.codproyecto803-C0-344
dc.identifier.codproyecto803-C1-509
dc.identifier.doi10.1016/j.btre.2022.e00780
dc.identifier.issn2215-017X
dc.identifier.urihttps://hdl.handle.net/10669/88167
dc.language.isoenges_ES
dc.rightsacceso abierto
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/*
dc.sourceBiotechnology reports 37 (2023)es_ES
dc.subjectSARS-CoV-2es_ES
dc.subjectRecombinant RBD expressiones_ES
dc.subjectFluorescent proteines_ES
dc.subjectMammalian cellses_ES
dc.subjectCOVID-19es_ES
dc.titleStable production of recombinant SARS-CoV-2 receptor-binding domain in mammalian cells with co-expression of a fluorescent reporter and its validation as antigenic target for COVID-19 serology testinges_ES
dc.typeartículo originales_ES

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