A metabolomic platform to identify and quantify polyphenols in coffee and related species using liquid chromatography mass spectrometry
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Fecha
2023
Tipo
artículo original
Autores
Castro Moretti, Fernanda R.
Cocuron, Jean Christophe
Castillo González, Humberto
Escudero Leyva, Efraín
Chaverri Echandi, Priscila
Guerreiro Filho, Oliveiro
Slot, Jason C.
Alonso, Ana Paula
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Introduction: Products of plant secondary metabolism, such as phenolic
compounds, flavonoids, alkaloids, and hormones, play an important role in
plant growth, development, stress resistance. The plant family Rubiaceae is
extremely diverse and abundant in Central America and contains several
economically important genera, e.g. Coffea and other medicinal plants.
These are known for the production of bioactive polyphenols (e.g. caffeine
and quinine), which have had major impacts on human society. The overall goal
of this study was to develop a high-throughput workflow to identify and
quantify plant polyphenols.
Methods: First, a method was optimized to extract over 40 families of
phytochemicals. Then, a high-throughput metabolomic platform has been
developed to identify and quantify 184 polyphenols in 15 min.
Results: The current metabolomics study of secondary metabolites was
conducted on leaves from one commercial coffee variety and two wild
species that also belong to the Rubiaceae family. Global profiling was
performed using liquid chromatography high-resolution time-of-flight mass
spectrometry. Features whose abundance was significantly different between
coffee species were discriminated using statistical analysis and annotated using
spectral databases. The identified features were validated by commercially available standards using our newly developed liquid chromatography tandem
mass spectrometry method.
Discussion: Caffeine, trigonelline and theobromine were highly abundant in
coffee leaves, as expected. Interestingly, wild Rubiaceae leaves had a higher
diversity of phytochemicals in comparison to commercial coffee: defenserelated molecules, such as phenylpropanoids (e.g., cinnamic acid), the
terpenoid gibberellic acid, and the monolignol sinapaldehyde were found
more abundantly in wild Rubiaceae leaves.
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Palabras clave
COFFEE, SPECIES, METABOLOGY, QUANTIFIER, CHROMATOGRAPHY