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Crystallization of the Lys49 PLA2 homologue, myotoxin II from the venom of Atropoides nummifer

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Watanabe, Leandra
Gava, Lisandra M.
Angulo Ugalde, Yamileth
Lomonte, Bruno
Arni, Raghuvir K.

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Abstract

Myotoxin II, a Lys49 catalytically inactive phospholipase A2 homologue from Atropoides nummifer venom, was purified, characterized and crystallized. The crystals belongs to the tetragonal system, space group P43212, with unit cell parameters (a=b=68.66 and c=63.87 2). Diffraction data were collected to a resolution of 2.32 2. The crystal structure is currently being determined using molecular replacement techniques.

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Keywords

Myotoxin, Snake venom, Phospholipase A2, Lysine-49, X-ray diffraction

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