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Exploration of immunoglobulin transcriptomes from mice immunized with three-finger toxins and phospholipases A2 from the Central American coral snake, Micrurus nigrocinctus

dc.creatorLaustsen, Andreas Hougaard
dc.creatorEngmark, Mikael Gerling
dc.creatorClouser, Christopher
dc.creatorTimberlake, Sonia
dc.creatorVigneault, Francois
dc.creatorGutiérrez, José María
dc.creatorLomonte, Bruno
dc.date.accessioned2017-02-20T19:40:19Z
dc.date.available2017-02-20T19:40:19Z
dc.date.issued2017
dc.description.abstractSnakebite envenomings represent a neglected public health issue in many parts of the rural tropical world. Animal-derived antivenoms have existed for more than a hundred years and are effective in neutralizing snake venom toxins when timely administered. However, the low immunogenicity of many small but potent snake venom toxins represents a challenge for obtaining a balanced immune response against the medically relevant components of the venom. Here, we employ high-throughput sequencing of the immunoglobulin (Ig) transcriptome of mice immunized with a three-finger toxin and a phospholipase A2 from the venom of the Central American coral snake, Micrurus nigrocinctus. Although exploratory in nature, our indicate results showed that only low frequencies of mRNA encoding IgG isotypes, the most relevant isotype for therapeutic purposes, were present in splenocytes of five mice immunized with 6 doses of the two types of toxins over 90 days. Furthermore, analysis of Ig heavy chain transcripts showed that no particular combination of variable (V) and joining (J) gene segments had been selected in the immunization process, as would be expected after a strong humoral immune response to a single antigen. Combined with the titration of toxin-specific antibodies in the sera of immunized mice, these data support the low immunogenicity of three-finger toxins and phospholipases A2 found in M. nigrocinctus venoms, and highlight the need for future studies analyzing the complexity of antibody responses to toxins at the molecular level.es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.citationhttps://peerj.com/articles/2924/
dc.identifier.doihttps://doi.org/10.7717/peerj.2924
dc.identifier.issn2167-8359
dc.identifier.issn2167-9843
dc.identifier.pmid28149694
dc.identifier.urihttps://hdl.handle.net/10669/29539
dc.language.isoen_USes_ES
dc.rightsacceso abierto
dc.rightsCC0 1.0 Universal*
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.sourcePeer Journal 5 (2017)es_ES
dc.subjectantivenomes_ES
dc.subjectantibodyes_ES
dc.subjectMicruruses_ES
dc.subjecttranscriptomicses_ES
dc.subjectSnake venomes_ES
dc.titleExploration of immunoglobulin transcriptomes from mice immunized with three-finger toxins and phospholipases A2 from the Central American coral snake, Micrurus nigrocinctuses_ES
dc.typeartículo original

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