Electrochemical biosensor for quantitative determination of fentanyl based on immobilized cytochrome c on multi-walled carbon nanotubes modified screen-printed carbon electrodes
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Fecha
2022
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artículo original
Autores
González Hernández, Jerson
Moya Alvarado, Guillermo
Alvarado Gámez, Ana Lorena
Urcuyo Solórzano, Roberto
Barquero Quirós, Miriam
Arcos Martínez, María Julia
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Fentanyl is a powerful synthetic opioid used to treat severe pain. New administration routes toward its illegal consumption
for recreational purposes pose a growing threat to public health, either due to misuse or abuse of this substance. As a result,
the rapid qualitative and quantitative determination of fentanyl in biofuids is of great interest. A novel enzymatic biosensor
based on adsorptive-stripping cyclic voltammetry is proposed as a cost-efective, reliable, and efcient device for fentanyl
determination in urine samples. Disposable screen-printed carbon electrodes modifed with multi-walled carbon nanotubes
and cytochrome c were used to develop the testing platform. The electrochemical behavior of fentanyl exhibited a welldefned anodic wave around 0.66 V vs. pseudo reference electrode. The experimental conditions were optimized to obtain
the best analytical response, and linear regression analysis of increasing concentration standards was applied to estimate
the performance parameters. The results suggest a simple method with a wide linearity range, high sensitivity, low limits
of detection (0.086 μg/mL) and quantifcation, and satisfactory precision (2.9% RSD). The feasibility and applicability of
the voltammetric approach were assessed by fentanyl-spiked urine samples by standard additions calibration curves in two
levels of enrichment with an accuracy of 92% and 100%.
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ELECTROCHEMISTRY, DRUGS, CARBON