Venom variation in Bothrops asper lineages from north-western South America.

Vista sencilla de ítem
dc.creatorMora Obando, Diana
dc.creatorSalazar Valenzuela, David
dc.creatorPla Ferrer, Davinia
dc.creatorLomonte, Bruno
dc.creatorGuerrero Vargas, Jimmy
dc.creatorAyerbe, Santiago
dc.creatorGibbs, H. Lisle
dc.creatorCalvete Chornet, Juan José
dc.date.accessioned2021-04-08T21:17:57Z
dc.date.available2021-04-08T21:17:57Z
dc.date.issued2020
dc.description.abstractBothrops asper is a venomous pitviper that is widely distributed and of clinical importance in Mesoamerica and northern South America, where it is responsible for 50–80% of all envenomations by Viperidae species. Previous work suggests that B. asper has a complex phylogeographic structure, with the existence of multiple evolutionarily distinct lineages, particularly in the inter-Andean valleys of north South America. To explore the impact of the evolutionary history of B. asper on venom composition, we have investigated geographic variation in the venom proteome of this species from the populations from the Pacific side of Ecuador and south-western Colombia. Among the 21 classes of venom components identified, proteins from mainly four major toxin families, snake venom metalloproteases (PI- and PII-SVMP), phospholipases A2 (K49- and D49-PLA2s), serine proteinases (SVSP), and C-type lectins-like (CTL) proteins are major contributors to the geographic variability in venom. Principal component analyses demonstrate significant differences in venom composition between B. asper lineages previously identified through combination of molecular, morphological and geographical data, and provide additional insights into the selection pressures modulating venom phenotypes on a geographic scale. In particular, altitudinal zonation within the Andean mountain range stands out as a key ecological factor promoting diversification in venom. In addition, the pattern of distribution of PLA2 molecules among B. asper venoms complements phylogenetic analysis in the reconstruction of the dispersal events that account for the current biogeographic distribution of the present-day species' phylogroups. Ontogenic variation was also evident among venoms from some Ecuadorian lineages, although this age-related variation was less extreme than reported in B. asper venoms from Costa Rica. The results of our study demonstrate a significant impact of phylogenetic history on venom composition in a pitviper and show how analyses of this variation can illuminate the timing of the cladogenesis and ecological events that shaped the current distribution of B. asper lineages.es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.sponsorshipMinisterio de Ciencia e Innovación/[BFU2017-89103-P]//Españaes_ES
dc.description.sponsorshipThe Ohio State University Graduate School/[]/OSU/Estados Unidoses_ES
dc.description.sponsorshipUniversidad Tecnológica Indoamérica/[]/UTI/Ecuadores_ES
dc.identifier.citationhttps://www.sciencedirect.com/science/article/abs/pii/S1874391920303134?via%3Dihub
dc.identifier.doi10.1016/j.jprot.2020.103945
dc.identifier.issn1874-3919
dc.identifier.urihttps://hdl.handle.net/10669/83193
dc.language.isoenges_ES
dc.rightsacceso embargado
dc.sourceJournal of Proteomics, vol.229, pp.1-19es_ES
dc.subjectSnake venomes_ES
dc.subjectVenomics of Bothrops asper lineageses_ES
dc.subjectPCA of intraspecific venom variationes_ES
dc.subjectBiogeography of B. asper venom radiationses_ES
dc.titleVenom variation in Bothrops asper lineages from north-western South America.es_ES
dc.typeartículo original

Archivos

Bloque original
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
2020_Prot_JProteomics_Mora_Bothrops asper_Lineages_Ecuador_Colombia.pdf
Tamaño:
9.02 MB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
2.83 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

Microbiología