Development of an instrument-free and low-cost ELISA dot-blot test to detect antibodies against SARS-CoV-2
Fecha
2023-02
Tipo
artículo original
Autores
Apú Leitón, Navilla
Madrigal Redondo, German
Herrera Vega, María
Corrales Aguilar, Eugenia
Segura Ulate, Ismael
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Resumen
Most laboratory tests to detect the presence of anti-SARS-CoV-2 antibodies use ELISA or CLIA; however, equipment for these immunoassays is unavailable in many areas of low- and middle-income countries. Rapid lateral flow immunoassay (LFIA) tests are a low-cost, equipment-free option, but their high price may make them less suitable for conducting seroprevalence surveys. Here, we describe a simple dual antigen ELISA dot-blot test to detect anti-SARS-CoV-2 IgG antibodies with high sensitivity (94 to 98%) and specificity (92 to 100%), compared to commercially available ELISA and CLIA options. Additionally, this ELISA dot-blot test can be completed in one hour using minimal laboratory equipment. Importantly, this immunoassay is significantly more affordable than most LFIA tests available on the global market. The dot-blot strips may be stored for up to 7 days under freezing conditions. This ELISA dot-blot test is a cost-effective option for conducting seroprevalence screenings in areas lacking ELISA or CLIA facilities, compared to LFIA tests.
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Palabras clave
SARS-CoV-2, COVID-19, antibodies, seroprevalence, ELISA dot-blot, IMMUNOLOGY