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Neutralization of myotoxic phospholipases A2 from the venom of the snake Bothrops asper by monoclonal antibodies

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dc.creatorLomonte, Bruno
dc.creatorGutiérrez, José María
dc.creatorRamírez Pacheco, Margarita
dc.creatorDíaz Oreiro, Cecilia
dc.date.accessioned2016-11-10T14:45:22Z
dc.date.available2016-11-10T14:45:22Z
dc.date.issued1992
dc.description.abstractThe neutralization of two myotoxic phospholipases A2 from the venom of Bothrops asper, myotoxins I and II, by two murine monoclonal antibodies is reported. The monoclonal antibodies, MAb-3 and Mab-4, recognize different epitopes of the toxin. Both antibodies completely neutralized myotoxic activity of myotoxin I and crude venom. MAb-3 also completely neutralized myotoxic activity of myotoxin II, a lysine-49 phospholipase A2 isoform, whereas MAb-4 neutralized this toxin only partially. MAb-3 neutralized myotoxin II at a molar ratio of 1:1, showing a higher efficiency than affinity-purified polyclonal antibodies. A dissociation of myotoxic and enzymatic activities of myotoxin I was observed with both monoclonal antibodies.es
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes
dc.identifier.doihttps://doi.org/10.1016/0041-0101(92)90866-4
dc.identifier.issn0040-0101
dc.identifier.urihttps://hdl.handle.net/10669/29173
dc.language.isoen_US
dc.rightsacceso embargado
dc.sourceToxicon. Volumen 30, Número 3, 1992es
dc.subjectAnimalses
dc.subjectAntibodieses
dc.subjectMonoclonales
dc.subjectCrotalid Venomses
dc.subjectEpitopeses
dc.subjectGroup II Phospholipases A2es
dc.subjectMicees
dc.subjectInbred BALB Ces
dc.subjectNeurotoxinses
dc.subjectNeutralization Testses
dc.subjectPhospholipases Aes
dc.subjectPhospholipases A2es
dc.subjectReptilian Proteinses
dc.titleNeutralization of myotoxic phospholipases A2 from the venom of the snake Bothrops asper by monoclonal antibodieses
dc.typeartículo original

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