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Neutralization of myotoxic phospholipases A2 from the venom of the snake Bothrops asper by monoclonal antibodies

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dc.creatorLomonte, Bruno
dc.creatorGutiérrez, José María
dc.creatorRamírez Pacheco, Margarita
dc.creatorDíaz Oreiro, Cecilia
dc.date.accessioned2016-11-10T14:45:22Z
dc.date.available2016-11-10T14:45:22Z
dc.date.issued1992
dc.description.abstractThe neutralization of two myotoxic phospholipases A2 from the venom of Bothrops asper, myotoxins I and II, by two murine monoclonal antibodies is reported. The monoclonal antibodies, MAb-3 and Mab-4, recognize different epitopes of the toxin. Both antibodies completely neutralized myotoxic activity of myotoxin I and crude venom. MAb-3 also completely neutralized myotoxic activity of myotoxin II, a lysine-49 phospholipase A2 isoform, whereas MAb-4 neutralized this toxin only partially. MAb-3 neutralized myotoxin II at a molar ratio of 1:1, showing a higher efficiency than affinity-purified polyclonal antibodies. A dissociation of myotoxic and enzymatic activities of myotoxin I was observed with both monoclonal antibodies.es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes_ES
dc.identifier.doi10.1016/0041-0101(92)90866-4
dc.identifier.issn0040-0101
dc.identifier.urihttps://hdl.handle.net/10669/29173
dc.language.isoen_USes_ES
dc.rightsacceso embargado
dc.sourceToxicon. Volumen 30, Número 3, 1992es_ES
dc.subjectAnimalses_ES
dc.subjectAntibodieses_ES
dc.subjectMonoclonales_ES
dc.subjectCrotalid Venomses_ES
dc.subjectEpitopeses_ES
dc.subjectGroup II Phospholipases A2es_ES
dc.subjectMicees_ES
dc.subjectInbred BALB Ces_ES
dc.subjectNeurotoxinses_ES
dc.subjectNeutralization Testses_ES
dc.subjectPhospholipases Aes_ES
dc.subjectPhospholipases A2es_ES
dc.subjectReptilian Proteinses_ES
dc.titleNeutralization of myotoxic phospholipases A2 from the venom of the snake Bothrops asper by monoclonal antibodieses_ES
dc.typeartículo original

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