Differential volatile organic compound expression in the interaction of Daldinia eschscholtzii and Mycena citricolor
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2023
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artículo original
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Escudero Leyva, Efraín
Quirós Guerrero, Luis Manuel
Vásquez Chaves, Víctor
Pereira Reyes, Reinaldo
Chaverri Echandi, Priscila
Tamayo Castillo, Giselle
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Resumen
Fungi exhibit a wide range of ecological guilds, but those that live within the inner
tissues of plants (a.k.a. endophytes) are particularly relevant due to the benefits they
sometimes provide to their hosts, such as herbivory deterrence, disease protection,
and growth promotion. Recently, endophytes have gained interest as potential
biocontrol agents against crop pathogens, for example, coffee plants (Coffea
arabica). Published results from research done in our laboratory showed that
endophytic fungi isolated from wild Rubiaceae plants were effective in reducing the
effects of the American Leaf Spot of Coffee (Mycena citricolor). One of these isolates
(GU11N) from the plant Randia grandifolia was identified as Daldinia eschscholtzii
(Xylariales). Its antagonism mechanisms, effects, and chemistry against M. citricolor
were investigated through the analysis of its volatile profile alone and in the presence
of the pathogen in contactless and dual culture assays. The experimental design
involved direct sampling of agar plugs in vials for headspace (HS) and headspacesolid-
phase-microextraction (HS-SPME) Gas Chromatography Mass Spectrometry
(GC-MS) analysis. Additionally, we used UHPLC-HRMS/MS to identify non-volatile
compounds from organic extracts of the mycelia involved in the interaction. Results
showed that more volatile compounds were identified using HS-SPME (39
components) than with the HS technique (13 components), sharing only 12
compounds. Statistical tests suggest that D. eschscholtzii inhibited the growth of M.
citricolor through the release of VOCs containing a combination of 1,8-
dimethoxynapththalene and terpene compounds affecting the M. citricolor
pseudopilei. The damaging effects of 1,8-dimethoxynaphthalene were corroborated
in an in vitro test against M. citricolor pseudopilei; SEM photographs confirmed the
structural damage. After analyzing the UHPLC-HRMS/MS data, a predominance of
fatty acid derivatives was found among the putatively identified compounds,
however, a considerable proportion of features (37.3%) remained unannotated. In
conclusion, our study suggests that D. eschscholtzii has a potential as a biocontrol
agent against M. citricolor and that 1,8-dimethoxynaphthalene contributes to the
observed damage to the pathogen’s reproductive structures.
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Palabras clave
FUNGI, COFFE PLANTS, PATHOLOGY, ORGANIC COMPOUNDS