Crystallization and preliminary X-ray diffraction analysis of suramin, a highly charged polysulfonated napthylurea, complexed with a myotoxic PLA2 from Bothrops asper venom
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Murakami, Mário T.
Gava, Lisandra M.
Zela, Sandro P.
Arruda, Emerson Z.
Melo, Paulo A.
Gutiérrez, José María
Arni, Raghuvir K.
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Abstract
Suramin is a highly charged polysulfonated napthylurea that interferes in a number of physiologically relevant processes such as myotoxicity, blood coagulation and several kinds of cancers. This synthetic compound was complexed with a myotoxic Lys49 PLA2 from Bothrops asper venom and crystallized by the hanging-drop vapor diffusion method at 18 °C. The crystals belong to the orthorhombic space group P212121, with unit cell parameters a=49.05, b=63.84 and c=85.67 Å. Diffraction data was collected to 1.78 Å.
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Keywords
Suramin, Myotoxicity, Lys49 PLA2s, X-Ray Diffraction, Snake venom