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Structure of a Lys49-Phospholipase A2 homologue isolated from the venom of Bothrops nummifer (jumping viper)

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dc.creatorde Azevedo, Walter Filgueira
dc.creatorWard, Richard John
dc.creatorGutiérrez, José María
dc.creatorArni, Raghuvir K.
dc.date.accessioned2017-01-23T15:44:26Z
dc.date.available2017-01-23T15:44:26Z
dc.date.issued1999-02
dc.description.abstractLys49-Phospholipase A2 (Lys49-PLA2) homologues damage membranes by a Ca2+-independent mechanism which does not involve catalytic activity. We have solved the structure of myotoxin-I, a Lys49-PLA2 homologue isolated from the venom of Bothrops nummifer (jumping viper) at 2.4 A resolution using molecular replacement techniques. The final model has been refined to a final R-factor of 18.4% (R-free = 23.2%), and shows excellent geometry. The myotoxin-I from Bothrops nummifer is dimeric in the crystalline state as has been observed for other Lys49-PLA2 homologues. In addition, a continuous electron density in the active site and substrate binding channel could be successfully modeled as a fatty-acid molecule.es
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo//FAPESP/Brasiles
dc.description.sponsorshipFundação para O Desenvolvimento da Unesp//FUNDUNESP/Brasiles
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico//CNPq/Brasiles
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010198001895
dc.identifier.doihttps://doi.org/10.1016/S0041-0101(98)00189-5
dc.identifier.issn0041-0101
dc.identifier.urihttps://hdl.handle.net/10669/29448
dc.language.isoen_US
dc.rightsacceso embargado
dc.sourceToxicon; Volumen 37, Número 2, 1999es
dc.subjectAnimalses
dc.subjectBinding Siteses
dc.subjectBothropses
dc.subjectCrystallizationes
dc.subjectDimerizationes
dc.subjectIn Vitro Techniqueses
dc.subjectLysinees
dc.subjectMolecular Conformationes
dc.subjectPalmitic Acides
dc.subjectPhospholipases Aes
dc.subjectPhospholipases A2es
dc.subjectProtein Conformationes
dc.subjectSensitivity and Specificityes
dc.subjectX-Ray Diffractiones
dc.subjectSnake venomes
dc.titleStructure of a Lys49-Phospholipase A2 homologue isolated from the venom of Bothrops nummifer (jumping viper)es
dc.typeartículo original

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