Begomovirus diversity in tomato crops in Costa Rica
Fecha
2023
Tipo
artículo original
Autores
Valverde Méndez, Karen
Hernández Jiménez, Eduardo José
Matamoros Rojas, Dennis
Barboza Vargas, Natalia María
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Resumen
Begomoviruses (Geminiviridae family) are characterized by their high recombination
rate and a wide range of hosts, making their control difficult. In Costa Rica, various
species of bipartite begomoviruses have been reported, which are Pepper golden
mosaic virus (PepGMV), Tomato yellow mottle virus (ToYMoV), Tomato leaf curl Sinaloa
virus (ToLCSiV) and the monopartite begomovirus Tomato yellow leaf curl virus
(TYLCV). Since the TYLCV first report in Costa Rica, neither additional knowledge
has been produced on how this begomovirus has spread in the country's territory nor
on the distribution of the other bipartite species. A total of 429 tomato samples
collected during the years 2015–2016 were used to study these aspects. Each
sample was georeferenced and analysed with various techniques such as nucleic acid
hybridization, polymerase chain reaction (PCR) and sequencing for the begomoviruses
previously reported in Costa Rica. It was found that the presence/absence of
the different species can vary, depending on the province. TYLCV is present in the
six provinces analysed in this work, with a proportion from 3.7 to 86.6 per cent. Alajuela,
Cartago, and Heredia are the provinces most affected by tomato-infecting
begomoviruses. Fourteen different haplotypes of TYLCV were detected, but all were
identified as TYLCV-IL. The distribution of TYLCV was related to the presence of the
whitefly Bemisia tabaci MED, especially in the country's main tomato production
areas. This information allows the phytosanitary surveillance services to develop
strategies for the integrated management of the disease and to contribute data to
the genetic improvement programmes of the crop.
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Palabras clave
TOMATO, CLONING, CULTIVATION, COSTA RICA