Potential of New Bacterial Strains for a Multiproduct Bioprocess Application: A Case Study Using Isolates of Lactic Acid Bacteria from Pineapple Silage of Costa Rican Agro-Industrial Residues
Fecha
2022-07-29
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artículo original
Autores
Montero Zamora, Jéssica Pamela
Rojas Vargas, María Daniela
Barboza Vargas, Natalia María
López Gómez, José Pablo
Mora Villalobos, José Anibal
Redondo Solano, Mauricio
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Resumen
Lactic acid bacteria (LAB) with potential for the development of multi-product processes
are necessary for the valorization of side streams obtained during the biotechnological production
of lactic acid (LA). In this study, 14 LAB strains isolated from pineapple agro-industrial residues in
Costa Rica were cultivated in microplates, and the six strains with the highest growth were selected
for fermentation in microbioreactors to evaluate the production of LA and acetic acid, and the
consumption of glucose. Lacticaseibacillus paracasei 6710 and L. paracasei 6714 presented the highest
OD600 values (1.600 and 1.602, respectively); however, the highest LA (in g/L) production was
observed in L. paracasei 6714 (14.50 ± 0.20) and 6712 (14.67 ± 0.42). L. paracasei 6714 was selected
for bioreactor fermentation and reached a maximum OD600 of 6.3062 ± 0.141, with a LA yield of
84.9% and a productivity of 1.06 g L−1 h
−1 after 21 h of fermentation. Finally, lipoteichoic acid (LTA)
detection from biomass was performed and the antimicrobial activity of the compounds present in
the supernatant was studied. LTA was detected from L. paracasei 6714 biomass, and its supernatant
caused significant inhibition of foodborne surrogate microorganisms. LAB isolated from pineapple
silage have biotechnological potential for multiproduct processes.
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bioproducts valorization, antimicrobial compounds, lipoteichoic acid, lactic acid, FERMENTATION, RECYCLING