Multidrug‑Resistant Escherichia coli in Costa Rican Domestic Wastewater Treatment Plants Maintains Horizontal Transfer Capacity of Resistance Determinants in Effluents
artículo original
Fecha
2023-06-13Autor
Rivera Montero, Luis César
Acuña Espínola, Gabriel
Barrantes Jiménez, Kenia
Rojas Jiménez, Keilor Osvaldo
Chacón Jiménez, Luz María
Metadatos
Mostrar el registro completo del ítemResumen
This study was conducted to evidence
the dissemination potential of antibiotic resistance
genetic elements in E. coli isolates of Costa Rican
domestic wastewater treatment plants (WWTPs). Few
studies have addressed this phenomenon in WWTPs
in Central America. Phenotypical resistance profiles
to β-lactams, quinolones, aminoglycosides, phenicols,
tetracyclines, and folate pathway inhibitors of
133 Escherichia coli isolates from the influent and
effluent of two urban WWTPs located in the Greater
Metropolitan Area of Costa Rica were described.
Thirty multidrug-resistant profiles were identified
and grouped into 15 genetic clones by ERIC-PCR; 6
of 15 genetic clones were from effluents. Six of the
seven examined genes (sulI, sulII, intI1, intI2, blaTEM,
and tetA) were found in multidrug-resistant isolates,
whereas blaOXA was absent. The horizontal gene conjugation
test confirmed the gene transfer capacity of
all tested isolates n = 8. Multidrug-resistant isolates in effluents with horizontal gene transfer capacity suggest
that Costa Rican WWTPs represent spots related
to antibiotic resistance spread to the environment. In
domestic WWTPs, we found that nearly 22% of E.
coli isolates presented a multidrug-resistant phenotype
capable of transferring their resistance determinants
by conjugation processes.
External link to the item
10.1007/s11270-023-06401-wColecciones
- Microbiología [1171]