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dc.creatorArias Arias, Jorge Luis
dc.creatorCorrales Aguilar, Eugenia
dc.creatorMora Rodríguez, Rodrigo Antonio
dc.date.accessioned2022-07-19T21:41:24Z
dc.date.available2022-07-19T21:41:24Z
dc.date.issued2021-06-22
dc.identifier.citationhttps://www.mdpi.com/journal/viruseses_ES
dc.identifier.issn1999-4915
dc.identifier.urihttps://hdl.handle.net/10669/86984
dc.description.abstractConventional plaque assays rely on the use of overlays to restrict viral infection allowing the formation of distinct foci that grow in time as the replication cycle continues leading to countable plaques that are visualized with standard techniques such as crystal violet, neutral red, or immuno- labeling. This classical approach takes several days until large enough plaques can be visualized and counted with some variation due to subjectivity in plaque recognition. Since plaques are clonal lesions produced by virus-induced cytopathic effect, we applied DNA fluorescent dyes with differen- tial cell permeability to visualize them by live-cell imaging. We could observe different stages of that cytopathic effect corresponding to an early wave of cells with chromatin-condensation followed by a wave of dead cells with membrane permeabilization within plaques generated by different animal viruses. This approach enables an automated plaque identification using image analysis to increase single plaque resolution compared to crystal violet counterstaining and allows its application to plaque tracking and plaque reduction assays to test compounds for both antiviral and cytotoxic activities. This fluorescent real-time plaque assay sums to those next-generation technologies by combining this robust classical method with modern fluorescence microscopy and image analysis approaches for future applications in virologyes_ES
dc.description.sponsorshipInternational Centre for Genetic Engineering and Biotechnology Grant CRP/CRI18-02.es_ES
dc.language.isoenges_ES
dc.sourceViruses, 13, 2021es_ES
dc.subjectvesicular stomatitises_ES
dc.subjectherpes simplexes_ES
dc.subjectyellow feveres_ES
dc.subjectanimal viruseses_ES
dc.subjectplaque assayes_ES
dc.subjectreal-timees_ES
dc.subjectlive-cell imaginges_ES
dc.subjectautomated image analysises_ES
dc.subjectDNA fluorescent dyeses_ES
dc.subjectantiviral screeninges_ES
dc.titleA Fluorescent Real-Time Plaque Assay Enables Single-Cell Analysis of Virus-Induced Cytopathic Effect by Live-Cell Imaginges_ES
dc.typeartículo científicoes_ES
dc.identifier.doihttps://doi.org/10.3390/v13071193
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES


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