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dc.creatorNikel Mayer, Pablo Iván
dc.creatorFuhrer, Tobias
dc.creatorChavarría Vargas, Max
dc.creatorSánchez Pascuala, Alberto
dc.creatorSauer, Uwe
dc.creatorde Lorenzo, Víctor
dc.date.accessioned2022-07-05T17:42:33Z
dc.date.available2022-07-05T17:42:33Z
dc.date.issued2021-01-11
dc.identifier.citationhttps://www.nature.com/articles/s41396-020-00884-9es_ES
dc.identifier.issn1751-7370
dc.identifier.urihttps://hdl.handle.net/10669/86894
dc.description.abstractAs a frequent inhabitant of sites polluted with toxic chemicals, the soil bacterium and plant-root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous oxidative stress. Yet, the ultimate reason of such phenotypic property remains largely unknown. To shed light on this question, metabolic network-wide routes for NADPH generation— the metabolic currency that fuels redox-stress quenching mechanisms—were inspected when P. putida KT2440 was challenged with a sub-lethal H2O2 dose as a proxy of oxidative conditions. 13C-tracer experiments, metabolomics, and flux analysis, together with the assessment of physiological parameters and measurement of enzymatic activities, revealed a substantial flux reconfiguration in oxidative environments. In particular, periplasmic glucose processing was rerouted to cytoplasmic oxidation, and the cyclic operation of the pentose phosphate pathway led to significant NADPH-forming fluxes, exceeding biosynthetic demands by ~50%. The resulting NADPH surplus, in turn, fueled the glutathione system for H2O2 reduction. These properties not only account for the tolerance of P. putida to environmental insults—some of which end up in the formation of reactive oxygen species—but they also highlight the value of this bacterial host as a platform for environmental bioremediation and metabolic engineering.es_ES
dc.description.sponsorshipThe Novo Nordisk Foundation///Noruegaes_ES
dc.description.sponsorshipEuropean Union’s Horizon 2020 Research and Innovation Programme/[814418]//Unión Europeaes_ES
dc.description.sponsorshipDanish Council for Independent Research///Dinamarcaes_ES
dc.description.sponsorshipMadonna University/[H2020-FET-OPEN-RIA-2017-1-766975]/MADONNA/Estados Unidoses_ES
dc.description.sponsorshipBioRoboost/[H2020-NMBP-BIO-CSA-2018]//Unión Europeaes_ES
dc.description.sponsorshipSynBio4Flav/[H2020-NMBP/0500]//Unión Europeaes_ES
dc.description.sponsorshipMix-Up: Contracts of European Union/[H2020-Grant 870294]/MIX-UP/Unión Europeaes_ES
dc.description.sponsorshipComunidad Autónoma de Madrid/[S2017/BMD-3691 InGEMICS-CM]//Españaes_ES
dc.language.isoenges_ES
dc.sourceThe ISME Journal; Núm. 15: 2021 pp. 1751-1766es_ES
dc.subjectPseudomonas putidaes_ES
dc.subjectBacteriumes_ES
dc.subjectBioremediationes_ES
dc.titleReconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stresses_ES
dc.typeartículo científicoes_ES
dc.identifier.doi10.1038/s41396-020-00884-9.
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones en Productos Naturales (CIPRONA)es_ES


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