Show simple item record

dc.creatorDurán Blanco, Gina
dc.creatorSolano Blanco, María Gabriela
dc.creatorGómez Argüello, Aarón
dc.creatorCordero Vásquez, Daniel
dc.creatorSánchez Sánchez, Adriana
dc.creatorVillalta Arrieta, Mauren
dc.creatorSánchez Chaves, Melvin
dc.creatorDíaz Oreiro, Cecilia
dc.creatorGutiérrez, José María
dc.creatorLeón Montero, Guillermo
dc.date.accessioned2022-05-05T19:06:38Z
dc.date.available2022-05-05T19:06:38Z
dc.date.issued2021-11
dc.identifier.citationwww.sciencedirect.com/science/article/pii/S2590171021000230es_ES
dc.identifier.issn2590-1710
dc.identifier.urihttps://hdl.handle.net/10669/86545
dc.description.abstractThe lethality neutralization assay in mice is the gold standard for the evaluation of the preclinical efficacy and specification fulfillment of snake antivenoms. However, owing to the animal suffering involved, this assay is a candidate to be replaced by in vitro alternatives or, at least, improved by the reduction of the number of animals used per experiment, the introduction of analgesia, and the refinement of the test. Since these tests are usually run for 24 or 48 h, one possibility to refine it is to shorten the endpoint observation time of the assay and so limiting the duration of suffering. To assess the effect of this modification of the standard procedure on the analytical properties of the assay, we compared the median lethal dose (LD50) and median effective dose (ED50) values, estimated through observation times of 6, 24 and 48 h. We used African and Latin American snake venoms and several batches of two polyspecific antivenoms. A significant correlation was found between LD50 and ED50 values estimated at the three observation times. Although some LD50 and ED50 values were significantly different at these time points, results of 6 h were robust enough to be used in the characterization of new antivenoms, the verification of specification compliance, and the parallel comparison of formulations. Our observations support the modification of the standard procedures used for assessing neutralizing ability of antivenoms by carrying out the observations at 6 h instead of 24 or 48 h, with the consequent reduction in the suffering inflicted upon mice during these assays. However, the shortening of the observation time in the lethality tests must be validated for each venom and antivenom before its introduction in the routine procedures.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A0-804]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-C0-523]/UCR/Costa Ricaes_ES
dc.description.sponsorshipWellcome Trust/[Reference 220517/Z/20/Z]//Reino Unidoes_ES
dc.language.isoenges_ES
dc.sourceToxicon: X, vol.12, pp.1-5.es_ES
dc.subjectAntivenom potencyes_ES
dc.subjectLethality neutralization assayes_ES
dc.subjectMedian effective dosees_ES
dc.subjectMedian lethal dosees_ES
dc.subjectReplacementes_ES
dc.subjectReductiones_ES
dc.subjectAnd refinement principlees_ES
dc.subjectSnake antivenomes_ES
dc.subjectsnake venomes_ES
dc.titleAssessing a 6-h endpoint observation time in the lethality neutralization assay used to evaluate the preclinical efficacy of snake antivenomses_ES
dc.typeartículo científicoes_ES
dc.identifier.doi10.1016/j.toxcx.2021.100087
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-A0-804
dc.identifier.codproyecto741-C0-523


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record