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dc.creatorRojas Azofeifa, Daniela
dc.creatorSasa Marín, Mahmood
dc.creatorLomonte, Bruno
dc.creatorDiego García, Elia
dc.creatorOrtiz Chaves, Natalia
dc.creatorBonilla Murillo, Fabián
dc.creatorMurillo Masís, Renato
dc.creatorTytgat, Jan
dc.creatorDíaz Oreiro, Cecilia
dc.date.accessioned2020-01-22T20:54:10Z
dc.date.available2020-01-22T20:54:10Z
dc.date.issued2019
dc.identifier.citationhttps://www.sciencedirect.com/science/article/abs/pii/S1532045618302126?via%3Dihub
dc.identifier.issn1532-0456
dc.identifier.urihttps://hdl.handle.net/10669/80365
dc.description.abstractnon-buthid venoms, for which knowledge is scarce. In this work, we characterized the venom of a Diplocentridae species, Didymocentrus krausi, a small fossorial scorpion that inhabits the Tropical Dry Forest of Central America. D. krausi venom soluble fraction contains proteases with enzymatic activity on gelatin and casein. Mass spectrometry and venomic analysis confirmed the presence of elastase-like, cathepsin-O-like proteases and a neprilysin- like metalloproteinase. We did not detect phospholipase A2, C or D, nor hyaluronidase activity in the venom. By homology-based venom gland transcriptomic analysis, NDBPs, a β-KTx-like peptide, and other putative toxin transcripts were found, which, together with a p-benzoquinone compound present in the venom, could potentially explain its direct hemolytic and cytotoxic effects in several mammalian cell lines. Cytotoxicity of D. krausi venom was higher than the effect of venoms from two buthid scorpion species distributed in Costa Rica, Centruroides edwardsii and Tityus pachyurus. Even though D. krausi venom was not lethal to mice or crickets, when injected in mouse gastrocnemius muscle at high doses it induced pathological effects at 24 h, which include myonecrosis, weak hemorrhage, and inflammatory infiltration. We observed an apparent thrombotic effect in the skin blood vessels, but no in vitro fibrinogenolytic activity was detected. In crickets, D. krausi venom induced toxicity and paralysis in short periods of timees_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B8-133]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A5-048]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceComparative Biochemistry and Physiology part C: Toxicology and Pharmacology C, vol.217, pp.54-67es_ES
dc.subjectScorpion venomes_ES
dc.subjectCytotoxicityes_ES
dc.subjectMyotoxicityes_ES
dc.subjectParalysises_ES
dc.subjectPeptideses_ES
dc.subjectToxinses_ES
dc.titleBiochemical characterization of the venom of Central American scorpion Didymocentrus krausi Francke, 1978 (Diplocentridae) and its toxic effects in vivo and in vitroes_ES
dc.typeartículo científico
dc.identifier.doi10.1016/j.cbpc.2018.11.021
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologíaes_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones en Productos Naturales (CIPRONA)es_ES
dc.identifier.codproyecto741-B8-133
dc.identifier.codproyecto741-A5-048


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