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dc.creatorVindas González, Julio
dc.creatorCarrera Rengifo, Yarina
dc.creatorLomonte, Bruno
dc.creatorGutiérrez, José María
dc.creatorCalvete Chornet, Juan José
dc.creatorSanz, Libia
dc.creatorFernández Ulate, Julián
dc.date.accessioned2019-01-30T17:19:20Z
dc.date.available2019-01-30T17:19:20Z
dc.date.issued2018-06-07
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S0141813018306019?via%3Dihub
dc.identifier.issn0141-8130
dc.identifier.urihttps://hdl.handle.net/10669/76514
dc.description.abstractThe first toxin isolated from the venomous pit viper Porthidiumophryomegas is a basic pentameric phospholipase A2 (PophPLA2). Elucidation of its amino acid sequence showed that it belongs to the group IIA of secreted PLA2s, with the presence of all 14 conserved cysteine positions. The toxin displayed catalytic activity, in agreementwith the presence of Asp49 in its sequence of 121 residues. SDS-PAGE analysis revealed that this toxin is pentameric in non-reducing conditions, a structural organization that has not been described for any viperid PLA2. PophPLA2 displayed moderate myotoxic (in vivo) and cytotoxic (in vitro) activities, as well as anticoagulant activity on human plasma (in vitro). PophPLA2 was not lethal, and did not induce signs of toxicity or distress in mice, when administered intravenously at a dose of up to 100 μg (5.9 μg/g). The toxin showed highest sequence identity with other PLA2s from the venoms of ancestral Asian pit viper species.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B5-602]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceInternational Journal of Biological Macromolecules, vol. 118, pp.1-8es_ES
dc.subjectSnakees_ES
dc.subjectVenomes_ES
dc.subjectMyotoxines_ES
dc.subjectPhospholipasees_ES
dc.subjectPorthidium ophryomegases_ES
dc.subjectVeneno de serpientees_ES
dc.subjectSnake venomes_ES
dc.titleA novel pentameric phospholipase A2 myotoxin (PophPLA2) from the venom of the pit viper Porthidium ophryomegases_ES
dc.typeartículo científico
dc.identifier.doi10.1016/j.ijbiomac.2018.06.028
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-B5-602


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