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A Novel Green Chemistry Method for Nonaqueous Extraction and High-Performance Liquid Chromatography Detection of First-, Second-, and Third-Generation Tetracyclines, 4-Epitetracycline, and Tylosin in Animal Feeds
dc.creator | Granados Chinchilla, Fabio | |
dc.creator | Sánchez González, Jorge Manuel | |
dc.creator | García Santamaría, Fernando | |
dc.creator | Rodríguez Sánchez, César | |
dc.date.accessioned | 2018-07-05T20:34:17Z | |
dc.date.available | 2018-07-05T20:34:17Z | |
dc.date.issued | 2012-06 | |
dc.identifier.citation | https://pubs.acs.org/doi/10.1021/jf301403f | |
dc.identifier.issn | 0021-8561 | |
dc.identifier.issn | 1520-5118 | |
dc.identifier.uri | https://hdl.handle.net/10669/75165 | |
dc.description.abstract | Although tetracyclines and macrolides are common additives for animal nutrition, methods for their simultaneous determination in animal feeds are nonexistent. By coupling an organic extraction and solid-phase extraction cleanup to a high-performance liquid chromatography separation and a nonaqueous postcolumn derivatization, we succeeded in detecting from 0.2 to 24.0 μg kg–1 of tetracycline, oxytetracycline, chlortetracycline, doxycycline, tigecycline, and 4-epitetracycline in this complex and heterogeneous matrix. Minocycline and tylosin could also be detected with our procedure, but using UV spectrophotometry (1.5 ≤ LOD ≤ 1.9 mg kg–1). Linear responses with correlation coefficients between 0.996 and 0.999 were obtained for all analytes in the 0.5–10 mg kg–1 concentration range. Average recoveries between 59 and 97% and between 98 and 102% were obtained for the tetracyclines and tylosin, respectively. Replicate standard deviations were typically below 5%. When this method was applied to 20 feeds marketed in Costa Rica, we detected labeling inconsistencies, banned mixtures of tetracyclines, and tetracycline concentrations that contravene international regulation. | es_ES |
dc.language.iso | en_US | es_ES |
dc.relation.ispartof | ||
dc.source | Journal of Agriculture and Food Chemistry, Vol 60(29), pp 7121–7128 | es_ES |
dc.subject | Animal feed | es_ES |
dc.subject | Green chemistry | es_ES |
dc.subject | HPLC | es_ES |
dc.subject | Tetracyclines | es_ES |
dc.subject | Tylosin | es_ES |
dc.subject | 636.084 Alimentación de animales | es_ES |
dc.title | A Novel Green Chemistry Method for Nonaqueous Extraction and High-Performance Liquid Chromatography Detection of First-, Second-, and Third-Generation Tetracyclines, 4-Epitetracycline, and Tylosin in Animal Feeds | es_ES |
dc.type | artículo original | |
dc.date.updated | 2018-06-06T14:57:29Z | |
dc.identifier.doi | 10.1021/jf301403f | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET) | es_ES |
dc.description.procedence | UCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiología | es_ES |
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