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dc.creatorChavarría Vargas, Max
dc.creatorGoñi Moreno, Ángel
dc.creatorde Lorenzo, Víctor
dc.creatorNikel Mayer, Pablo Iván
dc.description.abstractFructose uptake in the soil bacterium Pseudomonas putida occurs through a canonical phosphoenolpyruvate (PEP)-dependent sugar transport system (PTSFru). The logic of the genetic circuit that rules its functioning is puzzling: the transcription of the fruBKA operon, encoding all the components of PTSFru, can escape the repression exerted by the catabolite repressor/activator protein Cra solely in the presence of intracellular fructose-1-P, an agonist formed only when fructose has been already transported. To study this apparently incongruous regulatory architecture, the changes in the transcriptome brought about by a seamless Δcra deletion in P. putida strain KT2440 were inspected under different culture conditions. The few genes found to be upregulated in the cra mutant unexpectedly included PP_3443, encoding a bona fide glyceraldehyde-3-P dehydrogenase. An in silico model was developed to explore emergent properties that could result from such connections between sugar uptake with Cra and PEP. Simulation of fructose transport revealed that sugar uptake called for an extra supply of PEP (obtained through the activity of PP_3443) that was kept (i.e., memorized) even when the carbohydrate disappeared from the medium. This feature was traced to the action of two sequential inverters that connect the availability of exogenous fructose to intracellular PEP levels via Cra/PP_3443. The loss of such memory caused a much longer lag phase in cells shifted from one growth condition to another. The term “metabolic widget” is proposed to describe a merged biochemical and regulatory patch that tailors a given node of the cell molecular network to suit species-specific physiological needs.es_ES
dc.description.sponsorshipMinistry of Economy and Competitiveness/[RTC-2014-1777-3]/MINECO/Españaes_ES
dc.description.sponsorshipMinistry of Economy and Competitiveness/[BIO-2015-66960-C3-2-R]/MINECO/Españaes_ES
dc.description.sponsorshipEuropean Union/[EVOPROG (FP7-ICT-610730)]/UE/es_ES
dc.description.sponsorshipEuropean Union/[ARISYS (ERC-2012-ADG-322797)]/UE/es_ES
dc.description.sponsorshipEuropean Union/[EmPowerPutida (EU-H2020-BIOTEC-2014-2015-6335536]/UE/es_ES
dc.rightsAtribución 4.0 Internacional*
dc.sourceSystems, Vol. 1, Núm 6, 2016es_ES
dc.subjectCra regulatores_ES
dc.subjectMetabolic memoryes_ES
dc.subjectPTS sugar transportes_ES
dc.subjectPseudomonas putidaes_ES
dc.subjectGlyceraldehyde-3-P dehydrogenase,es_ES
dc.subjectNutrient shiftses_ES
dc.titleA Metabolic Widget Adjusts the Phosphoenolpyruvate-Dependent Fructose Influx in Pseudomonas putidaes_ES
dc.typeartículo científico
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones en Productos Naturales (CIPRONA)es_ES

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Atribución 4.0 Internacional
Except where otherwise noted, this item's license is described as Atribución 4.0 Internacional