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Lys-49 phospholipases A2 as active enzymes for beta-arachidonoyl phospholipid bilayer membranes
dc.creator | Yamaguchi, Yoko | |
dc.creator | Shimohigashi, Yasuyuki | |
dc.creator | Chiwata, Tsuyoshi | |
dc.creator | Tani, Ayako | |
dc.creator | Chijiwa, Takahisa | |
dc.creator | Lomonte, Bruno | |
dc.creator | Ohno, Motoroni | |
dc.date.accessioned | 2017-09-28T15:31:31Z | |
dc.date.available | 2017-09-28T15:31:31Z | |
dc.date.issued | 1997 | |
dc.identifier.citation | http://onlinelibrary.wiley.com/doi/10.1080/15216549700203771/abstract | |
dc.identifier.issn | 1039-9712 | |
dc.identifier.uri | https://hdl.handle.net/10669/73302 | |
dc.description.abstract | Phospholipases A2 containing Lys-49 have been reported to be extremely weak or inactive as enzyme. We have recently shown that basic proteins I and II (BP-I and BP-II), Lys- 49-PLA2s isolated from the venom of Trimeresurus flavoviridis (Habu snake), are potent to hydrolyze the arachidonate of 2-arachidonoyl-l-stearoyl-L-3-phosphatidylcholine (ASPC) in bilayer vesicles. In order to ensure such enzymatic activity of Lys-49-PLA2s, two other Lys-49- PLA2s from different snake venoms, myotoxin II (from Bothrops asper) and App-K49 (form Agkistrodon piscivorus piscivorus), were examined. Myotoxin II was found to be very active, even more potent than BP-II, liberating about 80% of arachidonic acid from liposomes. App-K49 was also active (about 50%) for ASPC liposomes. They were very weak or almost inactive for ASPC micelles and monomers. All these Lys-49-PLA2s were inactive for ASPC liposomes in the absence of Ca 2+. These results clearly demonstrated that Lys-49-PLA2s are the enzymes to hydrolyze the C2-ester bond of ASPC in bilayer membranes. | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Biochemistry and Molecular Biology International; Volumen 43, Número 1.1997 | es_ES |
dc.subject | Phospholipases A2 | es_ES |
dc.subject | Lys-49-phospholipases A2 | es_ES |
dc.subject | Lipolytic activity | es_ES |
dc.subject | Arachidonate | es_ES |
dc.title | Lys-49 phospholipases A2 as active enzymes for beta-arachidonoyl phospholipid bilayer membranes | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1080/15216549700203771 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
dc.identifier.pmid | 9315278 |
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