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Dengue Virus Infection of Primary Human Smooth Muscle Cells
(2018-11-05)
Dengue virus (DENV) infection of humans is presently the most important arthropod-borne viral global threat, for which no suitable or reliable animal model exists. Reports addressing the effect of DENV on vascular components ...
In Vitro Inhibition of Zika Virus Replication with Amantadine and Rimantadine Hydrochlorides
(2021-09-15)
Zika virus (ZIKV) is a mosquito-borne flavivirus in which human infection became relevant
during recent outbreaks in Latin America due to its unrecognized association with fetal neurological
disorders. Currently, there ...
Stable production of recombinant SARS-CoV-2 receptor-binding domain in mammalian cells with co-expression of a fluorescent reporter and its validation as antigenic target for COVID-19 serology testing
(2023-01)
SARS-CoV-2 receptor binding domain (RBD) recognizes the angiotensin converting enzyme 2 (ACE2) receptor in host cells that enables infection. Due to its antigenic specificity, RBD production is important for development ...
A Fluorescent Real-Time Plaque Assay Enables Single-Cell Analysis of Virus-Induced Cytopathic Effect by Live-Cell Imaging
(2021-06-22)
Conventional plaque assays rely on the use of overlays to restrict viral infection allowing
the formation of distinct foci that grow in time as the replication cycle continues leading to countable
plaques that are ...