Listar Microbiología por autor "dc5bee91-8304-4496-9bd9-957f59389841"
Mostrando ítems 1-20 de 49
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A Lys49-PLA2 myotoxin of Bothrops asper triggers a rapid death of macrophages that involves autocrine purinergic receptor signaling
Tonello, Fiorella; Simonato, M.; Aita, A.; Pizzo, Paola; Fernández Ulate, Julián; Lomonte, Bruno; Gutiérrez, José María; Montecucco, Cesare (Cell Death and Disease (2012) 3, e343, 2012-07-05)Lys49-PLA2 myotoxins, an important component of various viperid snake venoms, are a class of PLA2-homolog proteins deprived of catalytic activity. Similar to enzymatically active PLA2 (Asp49) and to other classes of ... -
A myotoxic Lys49 phospholipase A2-homologue is the major component of the venom of Bothrops cotiara from Misiones, Argentina
de Roodt, Adolfo Rafael; Fernández Ulate, Julián; Solano Centeno, Daniela; Lomonte, Bruno (2018-04-27)Bothrops cotiara is a pitviper found in Southeastern Brazil and, scarcely, in the Misiones province of Argentina. In contrast to considerable information available on the venom of the Brazilian snake population, that of ... -
A novel pentameric phospholipase A2 myotoxin (PophPLA2) from the venom of the pit viper Porthidium ophryomegas
Vindas González, Julio; Carrera Rengifo, Yarina; Lomonte, Bruno; Gutiérrez, José María; Calvete Chornet, Juan José; Sanz, Libia; Fernández Ulate, Julián (2018-06-07)The first toxin isolated from the venomous pit viper Porthidiumophryomegas is a basic pentameric phospholipase A2 (PophPLA2). Elucidation of its amino acid sequence showed that it belongs to the group IIA of secreted ... -
Amino acid sequence and biological characterization of BlatPLA2, a non-toxic acidic phospholipase A2 from the venom of the arboreal snake Bothriechis lateralis from Costa Rica
Van der Laat Villalobos, Marco; Fernández Ulate, Julián; Durban, Jordi; Villalobos Chacón, Eva; Camacho Umaña, Erika; Calvete Chornet, Juan José; Lomonte, Bruno (2013-10)Bothriechis is considered a monophyletic, basal genus of arboreal Neotropical pitvipers distributed across Middle America. The four species found in Costa Rica (B. lateralis, B. schlegeli, B. nigroviridis, B. supraciliaris) ... -
An interactive database for the investigation of high-density peptide microarray guided interaction patterns and antivenom cross-reactivity
Krause, Kamille E.; Jenkins, Timothy P.; Skaarup, Carina; Engmark, Mikael Mikael Gerling; Casewell, Nicholas R.; Ainsworth, Stuart; Lomonte, Bruno; Fernández Ulate, Julián; Gutiérrez, José María; Lund, Ole; Laustsen, Andreas Hougaard (2020)Snakebite envenoming is a major neglected tropical disease that affects millions of people every year. The only effective treatment against snakebite envenoming consists of unspecified cocktails of polyclonal antibodies ... -
Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activities
Antúnez Oliva, José; Fernández Ulate, Julián; Lomonte, Bruno; Angulo Ugalde, Yamileth; Sanz, Libia; Pérez, Alicia; Calvete Chornet, Juan José; Gutiérrez, José María (2010-09-30)Viperid snakes of the genus Atropoides are distributed in Mexico and Central America and, owing to their size and venom yield, are capable of provoking severe envenomings in humans. This study evaluated, using an ‘antivenomics’ ... -
Cell surface nucleolin interacts with and internalizes Bothrops asper Lys49 phospholipase A2 and mediates its toxic activity
Lina Massimino, Maria; Simonato, Morena; Spolaore, Barbara; Franchin, Cinzia; Arrigoni, Giorgio; Marin, Oriano; Monturiol Gross, Laura; Fernández Ulate, Julián; Lomonte, Bruno; Tonello, Fiorella (2018)Phospholipases A2 are a major component of snake venoms. Some of them cause severe muscle necrosis through an unknown mechanism. Phospholipid hydrolysis is a possible explanation of their toxic action, but catalytic and ... -
Characterization of a novel snake venom component: Kazal-type inhibitor-like protein from the arboreal pitviper Bothriechis schlegelii
Fernández Ulate, Julián; Gutiérrez, José María; Calvete Chornet, Juan José; Sanz, Libia; Lomonte, Bruno (2016-06)Snake venoms are composed mainly of a mixture of proteins and peptides. Notably, all snake venom toxins have been assigned to a small number of protein families. Proteomic studies on snake venoms have recently identified ... -
Combined venomics, venom gland transcriptomics, bioactivities, and antivenomics of two Bothrops jararaca populations from geographic isolated regions within the Brazilian Atlantic rainforest
Gonçalves Machado, Larissa; Pla Ferrer, Davinia; Sanz, Libia; Jorge, Roberta Jeane B.; Leitão De Araújo, Moema; Alves, Maria Lúcia M.; Alvares, Diego Janisch; De Miranda, Joari; Nowatzki, Jenifer; de Morais Zani, Karen; Fernandes, Wilson; Tanaka Azevedo, Anita Mitico; Fernández Ulate, Julián; Zingali, Russolina B.; Gutiérrez, José María; Corrêa Netto, Carlos; Calvete Chornet, Juan José (2016-03-01)Bothrops jararaca is a slender and semi-arboreal medically relevant pit viper species endemic to tropical and subtropical forests in southern Brazil, Paraguay, and northern Argentina (Misiones). Within its geographic range, ... -
Comparative biochemical characterization of Viperidae snake venoms from Perú reveals two compositional patterns of phospholipase A2 expression
Lomonte, Bruno; Díaz Oreiro, Cecilia; Chaves Mora, Fernando; Fernández Ulate, Julián; Ruiz, Marco; Salas, María; Zavaleta Martínez Vargas, Alfonso; Calvete Chornet, Juan José; Sasa Marín, Mahmood (2020)Snake species within the Bothrops complex (sensu lato) are of medical relevance in Latin America, but knowledge on their venom characteristics is limited, or even unavailable, for some taxa. Perú harbors 17 species of pit ... -
Comparative proteomic analysis of the venom of the taipan snake, Oxyuranus scutellatus, from Papua New Guinea and Australia: Role of neurotoxic and procoagulant effects in venom toxicity
Herrera Vega, María; Fernández Ulate, Julián; Vargas Arroyo, Mariángela; Villalta Arrieta, Mauren; Segura Ruiz, Álvaro; León Montero, Guillermo; Angulo Ugalde, Yamileth; Paiva, Owen K.; Matainaho, Teatulohi; Jensen, Simon D.; Winkel, Kenneth D.; Calvete Chornet, Juan José; Williams, David J.; Gutiérrez, José María (2012-04-03)The venom proteomes of populations of the highly venomous taipan snake, Oxyuranus scutellatus, from Australia and Papua New Guinea (PNG), were characterized by reverse-phase HPLC fractionation, followed by analysis of ... -
Cytotoxicity of snake venom Lys49 PLA2-like myotoxin on rat cardiomyocytes ex vivo does not involve a direct action on the contractile apparatus
López Dávila, Alfredo Jesús; Weber, Natalie; Kraft, Theresia; Matinmehr, Faramarz; Arias Hidalgo, Mariela Eugenia; Fernández Ulate, Julián; Lomonte, Bruno; Gutiérrez, José María (2021-09)Viperid snake venoms contain a unique family of cytotoxic proteins, the Lys49 PLA2 homologs, which are devoid of enzymatic activity but disrupt the integrity of cell membranes. They are known to induce skeletal muscle ... -
Envenomations by Bothrops and Crotalus Snakes Induce the Release of Mitochondrial Alarmins
Zornetta, Irene; Caccin, Paola; Fernández Ulate, Julián; Lomonte, Bruno; Gutiérrez, José María; Montecucco, Cesare (PLoS Negl Trop Dis 6(2): e1526, 2012-02-21)Skeletal muscle necrosis is a common manifestation of viperid snakebite envenomations. Venoms from snakes of the genus Bothrops, such as that of B. asper, induce muscle tissue damage at the site of venom injection, provoking ... -
Enzymatic labelling of venom phospholipase A2 toxins
Spolaore, Barbara; Fernández Ulate, Julián; Lomonte, Bruno; Massimino, Maria Lina; Tonello, Fiorella (2019)Almost all animal venoms contain secretory phospholipases A2 (PLA2s), 14 kDa disulfide-rich enzymes that hydrolyze membrane phospholipids at the sn-2 position, releasing lysophospholipids and fatty acids. These proteins, ... -
First crotoxin-like phospholipase A2 complex from a New World non-rattlesnake species: Nigroviriditoxin, from the arboreal Neotropical snake Bothriechis nigroviridis
Lomonte, Bruno; Mora Obando, Diana; Fernández Ulate, Julián; Sanz, Libia; Pla Ferrer, Davinia; Gutiérrez, José María; Calvete Chornet, Juan José (Toxicon vol 93:144–154, 2015-01)Bothriechis nigroviridis is an arboreal Neotropical pitviper found in Costa Rica and Panam a. A previous proteomic profiling of its venom revealed the presence of proteins with homology to the A and B subunits of ... -
First look into the venom of Roatan Island´s critically endangered coral snake Micrurus ruatanus: proteomic characterization, toxicity, immunorecognition and neutralization by an antivenom
Lippa, Enikő; Török, Ferenc; Gómez Argüello, Aarón; Corrales Chaves, Greivin; Chacón Gutiérrez, Danilo; Sasa Marín, Mahmood; Gutiérrez, José María; Lomonte, Bruno; Fernández Ulate, Julián (2019)A proteomic and toxicological study of the venom from one specimen of Micrurus ruatanus, a critically endangered coral snake species endemic to Roatan Island, Honduras, was carried out. Immunorecognition and neutralization ... -
Implementación de un laboratorio de análisis proteómicos en el Instituto Clodomiro Picado (UCR): primeras experiencias en Costa Rica
Lomonte, Bruno; Fernández Ulate, Julián (2012)Reseña sobre avances tecnológicos en la implementación de un laboratorio de análisis proteómico en Costa Rica. Se describe la espectrometría de masas, la ionización de muestra, la instrumentación básica, la espectrometría ... -
In vivo neutralization of Myotoxin II, a phospholipase A2 homolog from Bothrops asper venom, using peptides discovered via phage display technology
Laustsen, Andreas Hougaard; Gless, Bengt H.; Jenkins, Timothy P.; Meyhoff Madsen, Maria; Bjärtun, Johanna; Munk, Andreas S.; Oscoz, Saioa; Fernández Ulate, Julián; Gutiérrez, José María; Lomonte, Bruno; Lohse, Brian (2022)Many snake venom toxins cause local tissue damage in prey and victims, which constitutes an important pathology that is challenging to treat with existing antivenoms. One of the notorious toxins that causes such effects ... -
Integrative characterization of the venom of the coral snake Micrurus dumerilii (Elapidae) from Colombia: proteome, toxicity, and cross-neutralization by antivenom
Rey Suárez, Paola; Núñez Rangel, Vitelbina; Fernández Ulate, Julián; Lomonte, Bruno (2016-03)In Colombia, nearly 2.8% of the 4200 snakebite accidents recorded annually are inflicted by coral snakes (genus Micrurus). Micrurus dumerilii has a broad distribution in this country, especially in densely populated ... -
Intravascular hemolysis induced by phospholipases A2 from the venom of the Eastern coral snake, Micrurus fulvius: functional profile of hemolytic and non-hemolytic isoforms
Fernández Montes de Oca, María Laura; Lomonte, Bruno; Yunes Quartino, Pablo J.; Arce Bejarano, Ruth; Fernández Ulate, Julián; Camacho, Luis F.; Gutiérrez, José María; Kuemmel, Daniel; Fidelio, Gerardo Daniel (2018-04)A unique feature of the venom of Micrurus fulvius (Eastern coral snake) is its ability to induce severe intravascular hemolysis in particular species, such as dogs or mice. This effect was previously shown to be induced ...