Listar Microbiología por autor "6a9bac5f-130f-4977-8ddf-ab97fcafacc4"
Mostrando ítems 21-40 de 418
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A randomized double-blind clinical trial of two antivenoms in patients bitten by Bothrops atrox in Colombia
Otero Patiño, Rafael; Gutiérrez, José María; Núñez Rangel, Vitelbina; Robles, Abel; Estrada Umaña, Ricardo; Segura, Eduardo; Toro, María Fabiola; García, M. E.; Díaz, Abel; Ramírez, Eugenio; Gómez, G.; Castañeda, J.; Moreno, M. E. (1996-12)A randomized double-blind clinical trial in 39 patients envenomed by Bothrops atrox in Antioquia and Chocó, Colombia, was performed to compare the efficacy and safety of 2 equine-derived antivenoms prepared at Instituto ... -
A snake venom secreted phospholipase A2 induces foam cell formation depending on the activation of factors involved in lipid homeostasis
Leiguez, Elbio; do Nascimento Viana, Mariana; Hideki Matsubara, Márcio; Fernandes, Cristina Maria; Giannotti, Karina Cristina; Gutiérrez, José María; Lomonte, Bruno; Teixeira, Catarina de Fátima (2018-06)MT-III, a snake venom GIIA sPLA2, which shares structural and functional features with mammalian GIIA sPLA2s, activates macrophage defense functions including lipid droplet (LDs) formation, organelle involved in both lipid ... -
Ability of a polyvalent antivenom to neutralize the venom of Lachesis muta melanocephala, a new Costa Rican subspecies of the bushmaster
Gutiérrez, José María; Rojas Céspedes, Gustavo; Cerdas Fallas, Luis (1987)Several toxic and enzymatic activities of the venom of L. m. melanocephala were studied. This venom has many similarities with that of L. m. stenophrys, although there are quantitative differences in venom activities, as ... -
Ability of six Latin American antivenoms to neutralize the venom of mapaná equis (Bothrops atrox) from Antioquia and Chocó (Colombia)
Otero Patiño, Rafael; Núñez Rangel, Vitelbina; Osorio, Raúl Guillermo; Gutiérrez, José María; Giraldo, Cesar Augusto; Posada, Luz Elena (1995-06)This investigation compared the ability of six Latin American antivenoms (monovalent antibothropic INS, Santafé de Bogotá; polyvalent INS; polyvalent probiol, Santafé de Bogotá; antibothropic Instituto Butantan, IB, São ... -
Activation of cellular functions in macrophages by venom secretory Asp-49 and Lys-49 phospholipases A2
Zuliani, Juliana Pavan; Gutiérrez, José María; Casais e Silva, Luciana Lyra; Coccuzzo Sampaio, Sandra; Lomonte, Bruno; Teixeira, Catarina de Fátima (2005-10)The in vitro effects of myotoxin III (MT-III), an Asp-49 catalytically-active phospholipase A(2), and myotoxin II (MT-II), a catalytically-inactive Lys-49 variant, isolated from Bothrops asper snake venom, on phagocytosis ... -
La actividad proteolítica de los venenos de serpientes de Costa Rica sobre la caseína
Lomonte, Bruno; Gutiérrez, José María (Revista de Biologia Tropical 31(1), 37-40, 1983)Se establecen las condiciones óptimas para la determinación comparativa de la actividad proteolítica de los venenos de serpientes costarricenses sobre la caseína y se describe dicha actividad para 13 venenos diferentes. ... -
Activity of hemorrhagic metalloproteinase BaH-1 and myotoxin II from Bothrops asper snake venom on capillary endothelial cells in vitro
Lomonte, Bruno; Gutiérrez, José María; Borkow, Gadi; Ovadia, Michael; Tarkowski, Andrej; Hanson, Lars Ake (1994-04)In vivo, hemorrhagic toxins isolated from snake venoms cause a disorganization of the basal lamina of capillaries, with a concomitant degenerative process of endothelial cells. In this study we investigated the effects of ... -
Alteraciones en la microvasculatura y papel en la inflamación de la hemorragia pulmonar inducida por Csh1, una metaloproteinasa del veneno de la serpiente Crotalus simus
Castro Castro, Ana Cristina (2019-10)El envenenamiento inducido por mordeduras de serpiente afecta a millones de personas cada año, principalmente de poblaciones rurales vulnerables y la Organización Mundial de la Salud lo ha identificado como una enfermedad ... -
Amino acid sequence and crystal structure of BaP1, a metalloproteinase from Bothrops asper snake venom that exerts multiple tissue-damaging activities
Watanabe, Leandra; Shannon, John D.; Valente, Richard H.; Rucavado Romero, Alexandra; Alape Girón, Alberto; Kamiguti, Aura S.; Theakston, R. David G.; Fox, Jay W.; Gutiérrez, José María; Arni, Raghuvir K. (2003-10)BaP1 is a 22.7-kD P-I-type zinc-dependent metalloproteinase isolated from the venom of the snake Bothrops asper, a medically relevant species in Central America. This enzyme exerts multiple tissue-damaging activities, ... -
Amino acid sequence of a myotoxic Lys49-phospholipase A2 homologue from the venom of Cerrophidion (Bothrops) godmani
de Sousa, Marcelo Valle; Morhy, Lauro; Arni, Raghuvir K.; Ward, Richard John; Díaz Oreiro, Cecilia; Gutiérrez, José María (1998-05-19)The complete amino acid sequence of myotoxin II (godMT-II), a myotoxic phospholipase A2 (PLA2) homologue from the venom of the Central American crotaline snake Cerrophidion (Bothrops) godmani, was determined by direct ... -
An alternative in vitro method for testing the potency of the polyvalent antivenom produced in Costa Rica
Gutiérrez, José María; Ávila Agüero, Claudio; Cerdas Fallas, Luis; Rojas Umaña, Ermila; Cerdas Fallas, Luis (1988)The ability of several batches of polyvalent antivenom to neutralize indirect hemolytic activity of Bothrops asper venom was studied using a sensitive plate test. All samples of antivenom tested effectively neutralized ... -
An Asp49 Phospholipase A2 from Snake Venom Induces Cyclooxygenase-2 Expression and Prostaglandin E2 Production via Activation of NF-κB, p38MAPK, and PKC in Macrophages
Moreira, Vanessa; Lomonte, Bruno; Ramirez Vinolo, Marco Aurélio; Curi, Rui; Gutiérrez, José María; Teixeira, Catarina de Fátima (2014)Phospholipases A2 (PLA2) are key enzymes for production of lipid mediators. We previously demonstrated that a snake venom sPLA2 named MT-III leads to prostaglandin (PG)E2 biosynthesis in macrophages by inducing the expression ... -
An electrophoretic study on phospholipase A2 isoenzymes in the venoms of Central American crotaline snakes
Valiente Álvarez, Carmen Isabel; Moreno Robles, Edgardo; Sittenfeld Appel, Ana; Lomonte, Bruno; Gutiérrez, José María (1992)The number and isoelectric points of phospholipase A2 isoenzymes were studied in the venoms of 12 Central American crotaline snakes of the genera Bothrops, Crotalus, Lachesis and Agkistrodon. The study was carried out by ... -
An interactive database for the investigation of high-density peptide microarray guided interaction patterns and antivenom cross-reactivity
Krause, Kamille E.; Jenkins, Timothy P.; Skaarup, Carina; Engmark, Mikael Mikael Gerling; Casewell, Nicholas R.; Ainsworth, Stuart; Lomonte, Bruno; Fernández Ulate, Julián; Gutiérrez, José María; Lund, Ole; Laustsen, Andreas Hougaard (2020)Snakebite envenoming is a major neglected tropical disease that affects millions of people every year. The only effective treatment against snakebite envenoming consists of unspecified cocktails of polyclonal antibodies ... -
An MTT-based method for the in vivo quantification of myotoxic activity of snake venoms and its neutralization by antibodies
Lomonte, Bruno; Gutiérrez, José María; Romero, Marjorie; Núñez, Javier; Tarkowski, Andrej; Hanson, Lars Ake (1993-05)The reduction of the tetrazolium compound MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) was used as the basis for the development of a simple method for the quantitative estimation of metabolically ... -
Anti-human erythrocyte antibodies in horse-derived antivenoms used in the treatment of snakebite envenomations
León Montero, Guillermo; Rodríguez, Miguel Angel; Rucavado Romero, Alexandra; Lomonte, Bruno; Gutiérrez, José María (2007-03)This work examined the presence of antibodies reacting with human erythrocytes in horse-derived antivenoms used in the treatment of snakebite envenomations, and assessed the efficacy of various fractionation protocols in ... -
Antibody neutralization of a myotoxin from the venom of Bothrops asper (terciopelo)
Lomonte, Bruno; Gutiérrez, José María; Moreno Robles, Edgardo; Cerdas Fallas, Luis (1987)Neutralization of biological activities of B. asper myotoxin by a monospecific anti-myotoxin rabbit serum and polyvalent antivenom was studied. Both antisera neutralized myotoxic, phospholipase A and lethal activities of ... -
Antibody-mediated neutralization and binding-reversal studies on α-neurotoxins from micrurus nigrocinctus nigrocinctus (coral snake) venom
Alape Girón, Alberto; Stiles, Bradley G.; Gutiérrez, José María (1996-03)An ELISA based, non-radioactive acetylcholine receptor (AchR) binding assay was used to detect the alpha-neurotoxins present in Micrurus nigrocinctus nigrocinctus venom. Sera from horses hyperimmunized against M. nigrocinctus ... -
Anticomplementary activity of equine whole IgG antivenoms: comparison of three fractionation protocols
León Montero, Guillermo; Lomonte, Bruno; Gutiérrez, José María (2005-01)Early adverse reactions occur in a number of patients treated with heterologous antivenoms and have been associated with anticomplementary activity (ACA). In order to reduce the ACA of equine whole IgG antivenoms produced ... -
Anticomplementary Activity of Horse IgG and F(ab’)2 Antivenoms
Squaiella Baptistão, Carla Cristina; Marcelino, José Roberto; Ribeiro da Cunha, Luiz Eduardo; Gutiérrez, José María; Tambourgi, Denise V. (2014)Envenomation by poisonous animals is a neglected condition according to the World Health Organization (WHO). Antivenoms are included in the WHO Essential Medicines List. It has been assumed that immunoglobulin G (IgG) ...