Listar Microbiología por autor "3793c02e-dcc0-45b7-bc4a-2e5894feea14"
Mostrando ítems 1-20 de 32
-
A Clostridium difficile Lineage Endemic to Costa Rican Hospitals Is Multidrug Resistant by Acquisition of Chromosomal Mutations and Novel Mobile Genetic Elements
Ramírez Vargas, Gabriel; Quesada Gómez, Carlos; Acuña Amador, Luis Alberto; López Ureña, Diana; Murillo Corrales, Tatiana; Gamboa Coronado, María del Mar; Chaves Olarte, Esteban; Thomson, Nicholas R.; Rodríguez Cavallini, Evelyn; Rodríguez Sánchez, César (2017-01)The antimicrobial resistance (AMR) rates and levels recorded for Clostridium difficile are on the rise. This study reports the nature, levels, diversity, and genomic context of the antimicrobial resistance of human C. ... -
A MLST Clade 2 Clostridium difficile strain with a variant TcdB induces severe inflammatory and oxidative response associated with mucosal disruption
Leite Costa, Cecília; López Ureña, Diana; de Oliveira Assis, Thiago; Ribeiro, Ronaldo de Albuquerque; Silveira Silva, Rodrigo Otavio; Rupnik, Maja; Wilcox, Mark H.; Fiorini de Carvalho, Alex; Oliveira do Carmo, Anderson; Abalen Martins Dias, Adriana; Barreto Mano de Carvalho, Cibele; Chaves Olarte, Esteban; Rodríguez Sánchez, César; Quesada Gómez, Carlos; de Castro Brito, Gerly Anne (2016-08)The epidemiology of Clostridium difficile infections is highly dynamic as new strains continue to emerge worldwide. Here we present a detailed analysis of a new C. difficile strain (ICC-45) recovered from a cancer patient ... -
A Novel Green Chemistry Method for Nonaqueous Extraction and High-Performance Liquid Chromatography Detection of First-, Second-, and Third-Generation Tetracyclines, 4-Epitetracycline, and Tylosin in Animal Feeds
Granados Chinchilla, Fabio; Sánchez González, Jorge Manuel; García Santamaría, Fernando; Rodríguez Sánchez, César (2012-06)Although tetracyclines and macrolides are common additives for animal nutrition, methods for their simultaneous determination in animal feeds are nonexistent. By coupling an organic extraction and solid-phase extraction ... -
A single exposure of sediment sulphate-reducing bacteria to oxytetracycline concentrations relevant to aquaculture enduringly disturbed their activity, abundance and community structure
Fernández Montes de Oca, María Laura; Granados Chinchilla, Fabio; Rodríguez Sánchez, César (2015-05)Aim: although feed medicated with antibiotics is widely used in animal production to prevent and treat bacterial infections, the effect of these drugs on nontarget anaerobic bacteria is unknown. We aimed to clarify whether ... -
Analysis of TcdB Proteins within the Hypervirulent Clade 2 Reveals an Impact of RhoA Glucosylation on Clostridium difficile Proinflammatory Activities
Quesada Gómez, Carlos; López Ureña, Diana; Chumbler, Nicole; Kroh, Heather K.; Castro Peña, Carolina; Rodríguez Sánchez, César; Orozco Aguilar, Josué; González Camacho, Sara María; Rucavado Romero, Alexandra; Guzmán Verri, Caterina; Lawley, Trevor D.; Lacy, D. Borden; Chaves Olarte, Esteban (2016-03)Clostridium difficile strains within the hypervirulent clade 2 are responsible for nosocomial outbreaks worldwide. The increased pathogenic potential of these strains has been attributed to several factors but is still ... -
Avances de la bioinformática en Costa Rica: Vista retrospectiva y perspectivas
Campos Sánchez, Rebeca; Flores Cruz, Andrés; Molina Mora, José Arturo; Mora Rodríguez, Rodrigo Antonio; Rodríguez Sánchez, César; Gatica Arias, Andrés Mauricio; Guzmán Verri, Caterina (2021-11-09)Introducción: La disciplina científica de la bioinformática tiene el potencial de generar aplicaciones innovadoras para las sociedades humanas. Costa Rica, pequeña en tamaño y población en comparación con otros países de ... -
Bioavailability of in-feed tetracyclines is influenced to a greater extent by crude protein rather than calcium
Granados Chinchilla, Fabio; Rodríguez Sánchez, César (2014-12)Feed is a common vehicle for tetracycline administration in animal farming. However, veterinarians and farmers often overlook that common feed components, such as proteins and calcium, may interact with these drugs and ... -
Demonstration of antibiotic-induced tolerance development in tropical agroecosystems through physiological profiling of sediment microbial communities
Arias Andrés, María de Jesús; Ruepert, Clemens; García Santamaría, Fernando; Rodríguez Sánchez, César (2014-01)Agricultural use of antibiotics differs quantitatively and qualitatively in tropical and temperate countries. To gain insight into the nature and magnitude of physiological adaptations prompted by these drugs in microbial ... -
Distribution, stability, and protein interactions of Aflatoxin M1 in fresh cheese
Chavarría Molina, Guadalupe; Molina Alvarado, Andrea; Leiva Gabriel, Astrid; Méndez Hernández, Geovanna; Wong González, Eric; Cortés Muñoz, Marianela; Rodríguez Sánchez, César; Granados Chinchilla, Fabio (2017)Aflatoxin M1 (AFM1), a metabolite resulting from the hepatic metabolism of aflatoxin B1, is a potential carcinogen that can be found in milk, cheese, and others dairy products. In this work, we aimed to elucidate the ... -
Diversity and antimicrobial susceptibility of oxytetracycline-resistant isolates of Stenotrophomonas sp. and Serratia sp. associated with Costa Rican crops
Rodríguez Sánchez, César; Wachlin, A.; Altendorf, Karlheinz; García Santamaría, Fernando; Lipski, André (2007-08)Aims: to ameliorate the identification, evaluate the diversity, and determine the antimicrobial sensitivity of 19 oxytetracycline-resistant isolates of Stenotrophomonas sp. and Serratia sp. associated with Costa Rican ... -
Emergence of an Outbreak-Associated Clostridium difficile Variant with Increased Virulence
Quesada Gómez, Carlos; López Ureña, Diana; Chaves Olarte, Esteban; Acuña Amador, Luis Alberto; Villalobos Zúñiga, Manuel Antonio; Du, Tim; Freire, Rosemayre; Guzmán Verri, Caterina; Gamboa Coronado, María del Mar; Lawley, Trevor D.; Moreno Robles, Edgardo; Mulvey, Michael R.; de Castro Brito, Gerly Anne; Rodríguez Cavallini, Evelyn; Rodríguez Sánchez, César (2015-04)The prevalence of Clostridium difficile infections has increased due to the emergence of epidemic variants from diverse genetic lineages. Here we describe the emergence of a novel variant during an outbreak in a Costa Rican ... -
Emergence of Clostridium difficile NAP1 in Latin America
Quesada Gómez, Carlos; Rodríguez Sánchez, César; Gamboa Coronado, María del Mar; Rodríguez Cavallini, Evelyn; Du, Tim; Mulvey, Michael R.; Villalobos Zúñiga, Manuel Antonio; Boza Cordero, Ricardo (2010-02)The NAP1 and NAP2 strains of Clostridium difficile have been linked to nosocomial outbreaks of antibiotic-associated diarrhea (AAD) and pseudomembranous colitis in North American and European countries (4, 5). We found ... -
Exposure of a Tropical Soil to mg/kg of Oxytetracycline Elicits Hormetic Responses in the Catabolic Activities of its Microbial Community
Solís Jiménez, Yendry; Chavarría Molina, Guadalupe; García Santamaría, Fernando; Rodríguez Sánchez, César (2011-04)Many farmers in developing countries protect their crops with oxytetracycline and fertilize their farmlands with manure from animals that received this drug as growth promoter. In this study, a tropical soil was exposed ... -
High quality 3C de novo assembly and annotation of a multidrug resistant ST-111 Pseudomonas aeruginosa genome: Benchmark of hybrid and non-hybrid assemblers
Molina Mora, José Arturo; Campos Sánchez, Rebeca; Rodríguez Sánchez, César; Shi, Leming; García Santamaría, Fernando (2020)Genotyping methods and genome sequencing are indispensable to reveal genomic structure of bacterial species displaying high level of genome plasticity. However, reconstruction of genome or assembly is not straightforward ... -
Isolates of Clostridium perfringens recovered from Costa Rican patients with antibiotic-associated diarrhoea are mostly enterotoxin-negative and susceptible to first-choice antimicrobials
Camacho Matamoros, Natassia; Espinoza Aguirre, Juan Carlos; Rodríguez Sánchez, César; Rodríguez Cavallini, Evelyn (2008-03)To assess the prevalence of enterotoxigenic Clostridium perfringens among adults suffering from antibiotic-associated diarrhoea in a Costa Rican hospital, faecal samples were analysed from 104 patients by a cultivation ... -
Isolation of a Toxigenic and Clinical Genotype of Clostridium difficile in Retail Meats in Costa Rica
Quesada Gómez, Carlos; Mulvey, Michael R.; Vargas Dengo, Pablo Antonio; Gamboa Coronado, María del Mar; Rodríguez Sánchez, César; Rodríguez Cavallini, Evelyn (2013-02)We isolated a regional toxigenic genotype of Clostridium difficile, previously found in human infection in 4 of 200 (2%) samples of retail meats for human consumption: 1 of 67 samples of beef, 2 of 66 of pork, and 1 of 67 ... -
Lettuce for Human Consumption Collected in Costa Rica Contains Complex Communities of Culturable Oxytetracycline- and Gentamicin-Resistant Bacteria
Rodríguez Sánchez, César; Lang Lang, Lore; Wang Wong, Amy; Altendorf, Karlheinz; García Santamaría, Fernando; Lipski, André (2006-09)The present widespread use of antimicrobials in crop farming is based upon their successful application in human medicine. However, recent evidence suggests that the massive anthropogenic release of antimicrobials into ... -
Molecular detection of Clostridium difficile on inert surfaces from a Costa Rican hospital during and after an outbreak
Morales, Luisa; Rodríguez Sánchez, César; Gamboa Coronado, María del Mar (2016-12-01)Background: Hospital transmission of Clostridium difficile is fostered by contamination of surfaces and medical equipment with spores highly resistant to disinfectants and regular cleaning procedures. Despite the outbreaks ... -
Molecular epidemiology and antimicrobial resistance of Clostridium difficile in a national geriatric hospital in Costa Rica
Jiménez Solís, Andrea; Araya, R.; Paniagua Hidalgo, Diana; Camacho Mora, Zianne; Du, Tim; Golding, Geroge R.; Leandro Astorga, Gustavo; Rodríguez Sánchez, César; Quesada Gómez, Carlos (2018-08)Background: the epidemiology of Clostridium difficile infections (CDI) is changing rapidly worldwide; knowledge on the genotypes of C. difficile circulating in specialized geriatric hospitals and their resistance to ... -
Novel Clade C-I Clostridium difficile strains escape diagnostic tests, differ in pathogenicity potential and carry toxins on extrachromosomal elements
Ramírez Vargas, Gabriel; López Ureña, Diana; Badilla Lobo, Adriana; Orozco Aguilar, Josué; Murillo Corrales, Tatiana; Rojas, Priscilla; Riedel, Thomas; Overmann, Jörg; González, Gabriel; Chaves Olarte, Esteban; Quesada Gómez, Carlos; Rodríguez Sánchez, César (2018)The population structure of Clostridium difficile currently comprises eight major genomic clades. For the highly divergent C-I clade, only two toxigenic strains have been reported, which lack the tcdA and tcdC genes and ...