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A Novel Green Chemistry Method for Nonaqueous Extraction and High-Performance Liquid Chromatography Detection of First-, Second-, and Third-Generation Tetracyclines, 4-Epitetracycline, and Tylosin in Animal Feeds

Artículo científico
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Date
2012-06
Author
Granados Chinchilla, Fabio
Sánchez González, Jorge Manuel
García Santamaría, Fernando
Rodríguez Sánchez, César
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Abstract
Although tetracyclines and macrolides are common additives for animal nutrition, methods for their simultaneous determination in animal feeds are nonexistent. By coupling an organic extraction and solid-phase extraction cleanup to a high-performance liquid chromatography separation and a nonaqueous postcolumn derivatization, we succeeded in detecting from 0.2 to 24.0 μg kg–1 of tetracycline, oxytetracycline, chlortetracycline, doxycycline, tigecycline, and 4-epitetracycline in this complex and heterogeneous matrix. Minocycline and tylosin could also be detected with our procedure, but using UV spectrophotometry (1.5 ≤ LOD ≤ 1.9 mg kg–1). Linear responses with correlation coefficients between 0.996 and 0.999 were obtained for all analytes in the 0.5–10 mg kg–1 concentration range. Average recoveries between 59 and 97% and between 98 and 102% were obtained for the tetracyclines and tylosin, respectively. Replicate standard deviations were typically below 5%. When this method was applied to 20 feeds marketed in Costa Rica, we detected labeling inconsistencies, banned mixtures of tetracyclines, and tetracycline concentrations that contravene international regulation.
URI
http://hdl.handle.net/10669/75165
External link to the item
10.1021/jf301403f
https://pubs.acs.org/doi/10.1021/jf301403f
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  • Microbiología [879]



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  • Repositorios universitarios

  • Repositorio del SIBDI-UCR
  • Biblioteca Digital del CIICLA
  • Repositorio Documental Rafael Obregón Loría (CIHAC)
  • Biblioteca Digital Carlos Melendez (CIHAC)
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  • Colección de videos de UPA-VAS
  • Sitios recomendados

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  • Directory of Open Access Journals (DOAJ)
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Repositorio Institucional de la Universidad de Costa Rica. Algunos derechos reservados. Este repositorio funciona con DSpace.
Universidad de Costa Rica