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Open Table Philosophy: Establishment of the Integrated and End-of-Life Care Working Group in the Latin American Region
(2025) IFIC LatAm; Sáenz Madrigal, María del Rocío; Collado, Claudia; Pinto, Paula; Vacarezza Suazo, Cristóbal; Gómez Duarte, Ingrid; Sancho Rojas, Wilmer; Quesada Chaves, Jossel; López Vargas, Wendy; Solis Calvo, Luis Fernando
The document describes the creation of a collaborative, inclusive working group focused on integrated and end-of-life care tailored to Latin America’s unique social, cultural, and health contexts. It emphasizes a horizontal, open dialogue approach that values diverse perspectives, community participation, and ethical principles such as respect, dignity, autonomy, and social justice. The group aims to foster public policy development, interdisciplinary training, collaborative research, and the sharing of successful regional experiences to strengthen care practices in the region.
Longitudinal metabolomics and lipidomics analyses reveal alterations associated with envenoming by Bothrops asper and Daboia russelii in an experimental murine model
(2022-09-23) Wase, Nishikant; Gutiérrez, José María; Rucavado Romero, Alexandra; Fox, Jay W.
Longitudinal metabolomics and lipidomics analyses were carried out on the blood plasma of mice injected intramuscularly with venoms of the viperid species Bothrops asper or Daboia russelii. Blood samples were collected 1, 3, 6, and 24 h after venom injection, and a control group of non-envenomed mice was included. Significant perturbations in metabolomics and lipidomics were observed at 1, 3, and 6 h, while values returned close to those of control mice by 24 h, hence reflecting a transient pattern of metabolic disturbance. Both venoms induced significant changes in amino acids, as well as in several purines and pyrimidines, and in some metabolites of the tricarboxylic acid cycle. KEGG analysis of metabolic pathways that showed those with the greatest change included aminoacyl tRNA synthesis and amino acid biosynthesis and metabolism pathways. With regard to lipid metabolism, there was an increase in triglycerides and some acyl carnitines and a concomitant drop in the levels of some phospholipids. In addition, envenomed mice had higher levels of cortisol, heme, and some oxidative stress markers. The overall pattern of metabolic changes in envenomed mice bears similarities with the patterns described in several traumatic injuries, thus underscoring a metabolic response/adaptation to the injurious action of the venoms.
Why is skeletal muscle regeneration impaired after myonecrosis induced by viperid snake venoms?
(2018-05-01) Gutiérrez, José María; Escalante Muñoz, Teresa; Hernández, Rosario; Gastaldello, Stefano; Saravia Otten, Ingrid Patricia; Rucavado Romero, Alexandra
Skeletal muscle regeneration after myonecrosis involves the activation, proliferation and fusion of myogenic cells, and a coordinated inflammatory response encompassing phagocytosis of necrotic cell debris, and the concerted synthesis of cytokines and growth factors. Myonecrosis often occurs in snakebite envenomings. In the case of venoms that cause myotoxicity without affecting the vasculature, such as those of many elapid snakes, regeneration proceeds successfully. In contrast, in envenomings by most viperid snakes, which affect the vasculature and extracellular matrix in addition to muscle fibers, regeneration is largely impaired and, therefore, the muscle mass is reduced and replaced by fibro-adipose tissue. This review discusses possible causes for such poor regenerative outcome including: (a) damage to muscle microvasculature, which causes tissue hypoxia and affects the inflammatory response and the timely removal of necrotic tissue; (b) damage to intramuscular nerves, which results in atrophy of regenerating fibers; (c) degradation of muscle cell basement membrane, compromising the spatial niche for proliferating myoblasts; (d) widespread degradation of the extracellular matrix; and (e) persistence of venom components in the damaged tissue, which may affect myogenic cells at critical points in the regenerative process. Understanding the causes of poor muscle regeneration may pave the way for the development of novel therapeutic interventions aimed at fostering the regenerative process in envenomed patients.
Crystallization and preliminary diffraction data of BaP1, a haemorrhagic metalloproteinase from Bothrops asper snake venom
(2002) Watanabe, Leandra; Rucavado Romero, Alexandra; Kamiguti, Aura S.; Theakston, R. David G.; Gutiérrez, José María; Arni, Rughuvir K.
BaP1 is a metalloproteinase isolated from the venom of the Central American snake Bothrops asper (terciopelo). It is a 24 kDa protein consisting of a single chain which includes the metalloproteinase domain only, therefore being classified as a class P-I snake-venom metalloproteinase. BaP1 induces prominent local tissue damage, such as haemorrhage, myonecrosis, blistering, dermonecrosis and oedema. In order to elucidate its structure, BaP1 was crystallized by the hanging-drop vapour-diffusion technique in 0.1 M bicine pH 9.0, 10% PEG 20 000 and 2%(v/v) dioxane. Diffraction data were observed to a resolution of 2.7 Å. Crystals belong to space group P212121, with unit-cell parameters a = 38.22, b = 60.17, c = 86.09 Å.
Towards Building a Latin American Community on Integrated and End-of-Life Care
(2025) IFIC LatAm; Collado, Claudia; Pinto, Paula; Vacarezza Suazo, Cristóbal; Sáenz Madrigal, María del Rocío; Gómez Duarte, Ingrid; Sancho Rojas, Wilmer; Quesada Chaves, Jossel; López Vargas, Wendy; Solis Calvo, Luis Fernando
The presentation outlines the development of a Latin American Community of Practice focused on integrated care and end-of-life support, grounded in the open table philosophy. This space aims to connect diverse forms of knowledge, promote collaborative work among professionals, communities, and institutions, and strengthen regional networks. Key principles include community engagement, cultural relevance, shared governance, and knowledge exchange, with the goal of building collective capacities and context-sensitive experiences across the region.