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dc.creatorRucavado Romero, Alexandraes_ES
dc.creatorEscalante Muñoz, Teresaes_ES
dc.creatorShannon, John D.es_ES
dc.creatorAyala Castro, Carla N.es_ES
dc.creatorVillalta Arrieta, Maurenes_ES
dc.creatorGutiérrez, José Maríaes_ES
dc.creatorFox, Jay W.es_ES
dc.date.accessioned2017-02-27T21:47:11Zes_ES
dc.date.available2017-02-27T21:47:11Zes_ES
dc.date.issued2012es_ES
dc.identifier.citationhttp://pubs.acs.org/doi/abs/10.1021/pr200847qes_ES
dc.identifier.issn1535-3893es_ES
dc.identifier.urihttp://hdl.handle.net/10669/29564es_ES
dc.description2082-01 Embargo por política editoriales_ES
dc.description.abstractProteomic analysis of wound exudates represents a valuable tool to investigate tissue pathology and to assess the therapeutic success of various interventions. In this study, the ability of horse-derived IgG and F(ab0)2 antivenoms to neutralize local pathological effects induced by the venom of the snake Bothrops asper in mouse muscle was investigated by the proteomic analysis of exudates collected in the vicinity of affected tissue. In experiments involving the incubation of venom and antivenom prior to injection in mice, hemorrhagic activity was completely abolished and local muscle damaging activity was significantly reduced by the antivenoms. In these conditions, the relative amounts of several intracellular and extracellular matrix proteins were reduced by the action of antivenoms, whereas the relative amounts of various plasma proteins were not modified. Because not all intracellular proteins were reduced, it is likely that there is a residual cytotoxicity not neutralized by antivenoms. In experiments designed to more closely reproduce the actual circumstances of envenoming, that is, when antivenom is administered after envenomation, the number of proteins whose amounts in exudates were reduced by antivenoms decreased, underscoring the difficulty in neutralizing local pathology due to the very rapid onset of venom-induced pathology. In these experiments, IgG antivenom was more efficient than F(ab0)2 antivenom when administered after envenomation, probably as a consequence of differences in their pharmacokinetic profiles.en
dc.description.sponsorshipUniversidad de Costa Rica/[741-A9-003]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B0-528]/UCR/Costa Ricaes_ES
dc.description.sponsorshipNetwork for Research and Training in Tropical Diseases in Central America /[01-N-2010]/NeTropica/es_ES
dc.language.isoen_USes_ES
dc.sourceJournal of Proteome Research; Volumen 11, Número 1. 2012es_ES
dc.subjectSnake venomes_ES
dc.subjectBothrops asperes_ES
dc.subjectHemorrhagees_ES
dc.subjectMyonecrosises_ES
dc.subjectAntivenomes_ES
dc.subjectIgGes_ES
dc.subjectF(ab0)2es_ES
dc.subjectProteomicses_ES
dc.subjectWound exudatees_ES
dc.subjectExtracellular matrixes_ES
dc.titleEfficacy of IgG and F(ab′)2 Antivenoms to Neutralize Snake Venom-induced Local Tissue Damage as Assessed by the Proteomic Analysis of Wound Exudatees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1021/pr200847qes_ES
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-A9-003es_ES
dc.identifier.codproyecto741-B0-528es_ES
dc.identifier.pmid22004524es_ES


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